TY - JOUR
T1 - Natural transformation in Vibrio parahaemolyticus
T2 - A rapid method to create genetic deletions
AU - Chimalapati, Suneeta
AU - Santos, Marcela de Souza
AU - Servage, Kelly
AU - De Nisco, Nicole J.
AU - Dalia, Ankur B.
AU - Orth, Kim
N1 - Publisher Copyright:
© 2018 American Society for Microbiology.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - The Gram-negative bacterium Vibrio parahaemolyticus is an opportunistic human pathogen and the leading cause of seafood-borne acute gastroenteritis worldwide. Recently, this bacterium was implicated as the etiologic agent of a severe shrimp disease with consequent devastating outcomes to shrimp farming. In both cases, acquisition of genetic material via horizontal transfer provided V. parahaemolyticus with new virulence tools to cause disease. Dissecting the molecular mechanisms of V. parahaemolyticus pathogenesis often requires manipulating its genome. Classically, genetic deletions in V. parahaemolyticus are performed using a laborious, lengthy, multistep process. Here, we describe a fast and efficient method to edit this bacterium's genome based on V. parahaemolyticus natural competence. Although this method is similar to one previously described, V. parahaemolyticus requires counterselection for curing of acquired plasmids due to its recalcitrant nature of retaining extrachromosomal DNA. We believe this approach will be of use to the Vibrio community.
AB - The Gram-negative bacterium Vibrio parahaemolyticus is an opportunistic human pathogen and the leading cause of seafood-borne acute gastroenteritis worldwide. Recently, this bacterium was implicated as the etiologic agent of a severe shrimp disease with consequent devastating outcomes to shrimp farming. In both cases, acquisition of genetic material via horizontal transfer provided V. parahaemolyticus with new virulence tools to cause disease. Dissecting the molecular mechanisms of V. parahaemolyticus pathogenesis often requires manipulating its genome. Classically, genetic deletions in V. parahaemolyticus are performed using a laborious, lengthy, multistep process. Here, we describe a fast and efficient method to edit this bacterium's genome based on V. parahaemolyticus natural competence. Although this method is similar to one previously described, V. parahaemolyticus requires counterselection for curing of acquired plasmids due to its recalcitrant nature of retaining extrachromosomal DNA. We believe this approach will be of use to the Vibrio community.
KW - EspD
KW - Natural transformation systems
KW - T2SS
KW - Vibrio parahaemolyticus
UR - http://www.scopus.com/inward/record.url?scp=85049757231&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049757231&partnerID=8YFLogxK
U2 - 10.1128/JB.00032-18
DO - 10.1128/JB.00032-18
M3 - Article
C2 - 29555695
AN - SCOPUS:85049757231
SN - 0021-9193
VL - 200
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 15
M1 - e00032-18
ER -