Nature and distribution of feline sarcoma virus nucleotide sequences

A. E. Frankel, J. H. Gilbert, K. J. Porzig, E. M. Scolnick, S. A. Aaronson

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The genomes of three independent isolates of feline sarcoma virus (FeSV) were compared by molecular hybridization techniques. Using complementary DNAs prepared from two strains, SM- and ST-FeSV, common complementary DNAs were selected by sequential hybridization to FeSV and feline leukemia virus RNAs. These DNAs were shown to be highly related among the three independent virus isolates. FeSV specific complementary DNAs were prepared by selection for hybridization by the homologous FeSV RNA and against hybridization by feline leukemia virus RNA. Sarcoma virus-specific sequences of SM-FeSV were shown to differ from those of either ST- or GA-FeSV strains, whereas ST-FeSV-specific DNA shared extensive sequence homology with GA-FeSV. By molecular hybridization, each set of FeSV-specific sequences was demonstrated to be present in normal cat cellular DNA in approximately one copy per haploid genome and was conserved throughout Felidae. In contrast, FeSV-common sequences were present in multiple DNA copies and were found only in Mediterranean cats. The present results are consistent with the concept that each FeSV strain has arisen by a mechanism involving recombination between feline leukemia virus and cat cellular DNA sequences, the latter represented within the cat genome in a manner analogous to that of a cellular gene.

Original languageEnglish (US)
Pages (from-to)821-827
Number of pages7
JournalJournal of Virology
Volume30
Issue number3
StatePublished - 1979

Fingerprint

feline sarcoma viruses
Feline Sarcoma Viruses
nucleotide sequences
Feline Leukemia Virus
hybridization
Feline leukemia virus
complementary DNA
Cats
cats
Complementary DNA
DNA
Genome
RNA
genome
Viruses
viruses
Felidae
Haploidy
sarcoma

ASJC Scopus subject areas

  • Immunology

Cite this

Frankel, A. E., Gilbert, J. H., Porzig, K. J., Scolnick, E. M., & Aaronson, S. A. (1979). Nature and distribution of feline sarcoma virus nucleotide sequences. Journal of Virology, 30(3), 821-827.

Nature and distribution of feline sarcoma virus nucleotide sequences. / Frankel, A. E.; Gilbert, J. H.; Porzig, K. J.; Scolnick, E. M.; Aaronson, S. A.

In: Journal of Virology, Vol. 30, No. 3, 1979, p. 821-827.

Research output: Contribution to journalArticle

Frankel, AE, Gilbert, JH, Porzig, KJ, Scolnick, EM & Aaronson, SA 1979, 'Nature and distribution of feline sarcoma virus nucleotide sequences', Journal of Virology, vol. 30, no. 3, pp. 821-827.
Frankel AE, Gilbert JH, Porzig KJ, Scolnick EM, Aaronson SA. Nature and distribution of feline sarcoma virus nucleotide sequences. Journal of Virology. 1979;30(3):821-827.
Frankel, A. E. ; Gilbert, J. H. ; Porzig, K. J. ; Scolnick, E. M. ; Aaronson, S. A. / Nature and distribution of feline sarcoma virus nucleotide sequences. In: Journal of Virology. 1979 ; Vol. 30, No. 3. pp. 821-827.
@article{5c0306b979df4f33a391e3ca497b2c90,
title = "Nature and distribution of feline sarcoma virus nucleotide sequences",
abstract = "The genomes of three independent isolates of feline sarcoma virus (FeSV) were compared by molecular hybridization techniques. Using complementary DNAs prepared from two strains, SM- and ST-FeSV, common complementary DNAs were selected by sequential hybridization to FeSV and feline leukemia virus RNAs. These DNAs were shown to be highly related among the three independent virus isolates. FeSV specific complementary DNAs were prepared by selection for hybridization by the homologous FeSV RNA and against hybridization by feline leukemia virus RNA. Sarcoma virus-specific sequences of SM-FeSV were shown to differ from those of either ST- or GA-FeSV strains, whereas ST-FeSV-specific DNA shared extensive sequence homology with GA-FeSV. By molecular hybridization, each set of FeSV-specific sequences was demonstrated to be present in normal cat cellular DNA in approximately one copy per haploid genome and was conserved throughout Felidae. In contrast, FeSV-common sequences were present in multiple DNA copies and were found only in Mediterranean cats. The present results are consistent with the concept that each FeSV strain has arisen by a mechanism involving recombination between feline leukemia virus and cat cellular DNA sequences, the latter represented within the cat genome in a manner analogous to that of a cellular gene.",
author = "Frankel, {A. E.} and Gilbert, {J. H.} and Porzig, {K. J.} and Scolnick, {E. M.} and Aaronson, {S. A.}",
year = "1979",
language = "English (US)",
volume = "30",
pages = "821--827",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Nature and distribution of feline sarcoma virus nucleotide sequences

AU - Frankel, A. E.

AU - Gilbert, J. H.

AU - Porzig, K. J.

AU - Scolnick, E. M.

AU - Aaronson, S. A.

PY - 1979

Y1 - 1979

N2 - The genomes of three independent isolates of feline sarcoma virus (FeSV) were compared by molecular hybridization techniques. Using complementary DNAs prepared from two strains, SM- and ST-FeSV, common complementary DNAs were selected by sequential hybridization to FeSV and feline leukemia virus RNAs. These DNAs were shown to be highly related among the three independent virus isolates. FeSV specific complementary DNAs were prepared by selection for hybridization by the homologous FeSV RNA and against hybridization by feline leukemia virus RNA. Sarcoma virus-specific sequences of SM-FeSV were shown to differ from those of either ST- or GA-FeSV strains, whereas ST-FeSV-specific DNA shared extensive sequence homology with GA-FeSV. By molecular hybridization, each set of FeSV-specific sequences was demonstrated to be present in normal cat cellular DNA in approximately one copy per haploid genome and was conserved throughout Felidae. In contrast, FeSV-common sequences were present in multiple DNA copies and were found only in Mediterranean cats. The present results are consistent with the concept that each FeSV strain has arisen by a mechanism involving recombination between feline leukemia virus and cat cellular DNA sequences, the latter represented within the cat genome in a manner analogous to that of a cellular gene.

AB - The genomes of three independent isolates of feline sarcoma virus (FeSV) were compared by molecular hybridization techniques. Using complementary DNAs prepared from two strains, SM- and ST-FeSV, common complementary DNAs were selected by sequential hybridization to FeSV and feline leukemia virus RNAs. These DNAs were shown to be highly related among the three independent virus isolates. FeSV specific complementary DNAs were prepared by selection for hybridization by the homologous FeSV RNA and against hybridization by feline leukemia virus RNA. Sarcoma virus-specific sequences of SM-FeSV were shown to differ from those of either ST- or GA-FeSV strains, whereas ST-FeSV-specific DNA shared extensive sequence homology with GA-FeSV. By molecular hybridization, each set of FeSV-specific sequences was demonstrated to be present in normal cat cellular DNA in approximately one copy per haploid genome and was conserved throughout Felidae. In contrast, FeSV-common sequences were present in multiple DNA copies and were found only in Mediterranean cats. The present results are consistent with the concept that each FeSV strain has arisen by a mechanism involving recombination between feline leukemia virus and cat cellular DNA sequences, the latter represented within the cat genome in a manner analogous to that of a cellular gene.

UR - http://www.scopus.com/inward/record.url?scp=0018746687&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018746687&partnerID=8YFLogxK

M3 - Article

VL - 30

SP - 821

EP - 827

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 3

ER -