Neuronal (type I) nitric oxide synthase regulates nuclear factorκb activity and immunologic (type II) nitric oxide synthase expression

H. Togashi, M. Sasaki, Elliot Frohman, E. Taira, R. R. Ratan, T. M. Dawson, V. L. Dawson

Research output: Contribution to journalArticle

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Abstract

Nitric oxide subserves diverse physiologic roles in the nervous system. NO is produced from at least three different NO synthase (NOS) isoforms: neuronal NOS (nNOS), endothelial NOS, and immunologic NOS (iNOS). We show that nNOS is the predominant isoform constitutively expressed in glia. NO derived from nNOS in glia inhibits the transcription factor nuclear factor κB (NFκB) as NOS inhibitors enhance basal NFκB activation. Pyrrolidine dithiocarbamate (PDTC) is an inhibitor of NFκB in most cells; however, we show that PDTC is also a potent scavenger of NO through formation of mononitrosyl iron complexes with PDTC. In Jurkat cells, a human T-cell lymphoma cell line, tumor necrosis factor-α (TNF-α) induces NFκB activation that is inhibited by PDTC. Contrary to the results in Jurkat cells, PDTC did not inhibit tumor necrosis factor-α-induced NFκB activation in astrocytes; instead PDTC itself induces NFκB activation in astrocytes, and this may be related to scavenging of endogenously produced NO by the PDTC iron complex. In astrocytes PDTC also dramatically induces the NFκB- dependent enzyme, iNOS, supporting the physiologic relevance of endogenous NO regulation of NFκB. NFκB activation in gila from mice lacking nNOS responds more rapidly to PDTC compared with astrocytes from wild-type mice. Our data suggest that nNOS in astrocytes regulates NFκB activity and iNOS expression, and indicate a novel regulatory role for nNOS in tonically suppressing central nervous system, NFκB-regulated genes.

Original languageEnglish (US)
Pages (from-to)2676-2680
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number6
DOIs
Publication statusPublished - 1997

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ASJC Scopus subject areas

  • Genetics
  • General

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