NG2 cells are not a major source of reactive astrocytes after neocortical stab wound injury

Mila Komitova, David R. Serwanski, Q. Richard Lu, Akiko Nishiyama

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

NG2 cells are an abundant glial cell type in the adult brain. They are distinct from astrocytes, mature oligodendrocytes, and microglia. NG2 cells generate oligodendrocytes and a subpopulation of protoplasmic astrocytes in the ventral forebrain during development. To determine whether NG2 cells generate reactive astrocytes in the lesioned brain, stab wound injury was created in adult NG2creBAC:ZEG double transgenic mice, in which enhanced green fluorescent protein (EGFP) is expressed in NG2 cells and their progeny, and the phenotype of the EGFP+ cells was analyzed at 10 and 30 days post lesion (dpl). The majority (>90%) of the reactive astrocytes surrounding the lesion that expressed glial fibrillary acidic protein (GFAP) lacked EGFP expression, and conversely the majority (>90%) of EGFP+ cells were GFAP-negative. However, 8% of EGFP+ cells co-expressed GFAP at 10 dpl. Most of these EGFP+GFAP+ cells were morphologically distinct from hypertrophic reactive astrocytes and exhibited weak GFAP expression. NG2 was detected in a fraction of the EGFP+GFAP+ cells found at 10 dpl. By 30 dpl the number of EGFP+GFAP+ cells had decreased more than four-fold from 10 dpl. A similar transient appearance of EGFP+GFAP+ cells with simple morphology was observed in NG2creER™:ZEG double transgenic mice in which EGFP expression had been induced in NG2 cells prior to injury. NG2 cell-specific deletion of the oligodendrocyte lineage transcription factor Olig2 using NG2creER™:Olig2fl/fl:ZEG triple transgenic mice did not increase the number of EGFP+ reactive astrocytes. These findings suggest that NG2 cells are not a major source of reactive astrocytes in the neocortex.

Original languageEnglish (US)
Pages (from-to)800-809
Number of pages10
JournalGLIA
Volume59
Issue number5
DOIs
StatePublished - May 2011

Fingerprint

Stab Wounds
Astrocytes
Glial Fibrillary Acidic Protein
Wounds and Injuries
Oligodendroglia
Transgenic Mice
enhanced green fluorescent protein
Neocortex
Brain
Microglia
Prosencephalon
Neuroglia

Keywords

  • NG2
  • Oligodendrocyte
  • Reactive astrocyte

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Komitova, M., Serwanski, D. R., Richard Lu, Q., & Nishiyama, A. (2011). NG2 cells are not a major source of reactive astrocytes after neocortical stab wound injury. GLIA, 59(5), 800-809. https://doi.org/10.1002/glia.21152

NG2 cells are not a major source of reactive astrocytes after neocortical stab wound injury. / Komitova, Mila; Serwanski, David R.; Richard Lu, Q.; Nishiyama, Akiko.

In: GLIA, Vol. 59, No. 5, 05.2011, p. 800-809.

Research output: Contribution to journalArticle

Komitova, M, Serwanski, DR, Richard Lu, Q & Nishiyama, A 2011, 'NG2 cells are not a major source of reactive astrocytes after neocortical stab wound injury', GLIA, vol. 59, no. 5, pp. 800-809. https://doi.org/10.1002/glia.21152
Komitova, Mila ; Serwanski, David R. ; Richard Lu, Q. ; Nishiyama, Akiko. / NG2 cells are not a major source of reactive astrocytes after neocortical stab wound injury. In: GLIA. 2011 ; Vol. 59, No. 5. pp. 800-809.
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