Nitric oxide mediates increases in skin blood flow during local warming in humans

D. L. Kellogg, Y. Liu, I. F. Kosiba, J. M. Johnson, C. G. Crandall, D. O'Donnell, A. M M Shepherd

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Local warming of skin induces vasodilation by unknown mechanisms. To test whether nitric oxide(NO) is required for such increases in skin blood flow(SkBF), we examined effects of NO-synthase(NOS) inhibition with L-nitro-arginine methylester (L-NAME) on vasodilation induced by local warming of skin in 5 subjects. Two adjacent sites on the forearm were instrumented with intradermal microdialysis probes for local delivery of L-NAME or Nitroprusside. SkBF was monitored by laser-Doppler flowmetry(LDF) at microdialysis sites. Local temperature(Tloc) at both sites was controlled with special LDF probe holders. Mean arterial pressure(MAP, Finapres) was measured and cutaneous vascular conductance calculated(CVC=LDF/MAP). Data collection began with a 10 minute control periodá(Tloc at both sites=34°C). One site was then warmed to 41°C while the second was maintained at 34°C throughout the study. Local warming of the skin increased CVC by 377±136%(p<0.01). L-NAME administration reduced CVC to 217±62% of control(p>0.05 vs control, p<0.05 vs heating), despite the elevation of Tloc. Subsequent administration of Nitroprusside increased CVC to levels no different than those induced by local warming alone. At a Tloc of 34°, L-NAME had no significant effect on CVC; however, Nitroprusside administration increased CVC by 443±57%(p<0.01). Thus, NOS inhibition reduced, and Nitroprusside restored, the cutaneous vasodilation induced by elevation of Tloc. We conclude that the mechanism of vasodilation by local warming of the skin requires NO generation by NOS.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

Fingerprint

skin (animal)
blood flow
nitric oxide
Skin
Nitric Oxide
Blood
vasodilation
Nitroprusside
Vasodilation
Nitric Oxide Synthase
Laser-Doppler Flowmetry
lasers
arginine
Arginine
probes (equipment)
Lasers
Microdialysis
nitric oxide synthase
blood vessels
Forearm

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Kellogg, D. L., Liu, Y., Kosiba, I. F., Johnson, J. M., Crandall, C. G., O'Donnell, D., & Shepherd, A. M. M. (1997). Nitric oxide mediates increases in skin blood flow during local warming in humans. FASEB Journal, 11(3).

Nitric oxide mediates increases in skin blood flow during local warming in humans. / Kellogg, D. L.; Liu, Y.; Kosiba, I. F.; Johnson, J. M.; Crandall, C. G.; O'Donnell, D.; Shepherd, A. M M.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Kellogg, DL, Liu, Y, Kosiba, IF, Johnson, JM, Crandall, CG, O'Donnell, D & Shepherd, AMM 1997, 'Nitric oxide mediates increases in skin blood flow during local warming in humans', FASEB Journal, vol. 11, no. 3.
Kellogg DL, Liu Y, Kosiba IF, Johnson JM, Crandall CG, O'Donnell D et al. Nitric oxide mediates increases in skin blood flow during local warming in humans. FASEB Journal. 1997;11(3).
Kellogg, D. L. ; Liu, Y. ; Kosiba, I. F. ; Johnson, J. M. ; Crandall, C. G. ; O'Donnell, D. ; Shepherd, A. M M. / Nitric oxide mediates increases in skin blood flow during local warming in humans. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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