Nitric oxide synthase isoform expression in the developing lung epithelium

T. S. Sherman, Z. Chen, I. S. Yuhanna, K. S. Lau, L. R. Margraf, P. W. Shaul

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Nitric oxide (NO), generated by NO synthase (NOS), is an important mediator of physiological processes in the airway and lung parenchyma, and there is evidence that the pulmonary expression of the endothelial isoform of NOS (eNOS) is developmentally regulated. The purpose of the present study was to delineate the cellular distribution of expression of eNOS in the developing respiratory epithelium and to compare it with inducible (iNOS) and neuronal (nNOS) NOS. Immunohistochemistry was performed on fetal (125-135 days gestation, term 144 days), newborn (2-4 wk), and maternal sheep lungs. In fetal lung, eNOS expression was evident in bronchial and proximal bronchiolar epithelia but was absent in terminal and respiratory bronchioles and alveolar epithelium. Similar to eNOS, iNOS was detected in bronchial and proximal bronchiolar epithelia but not in alveolar epithelium. However, iNOS was also detected in terminal and respiratory bronchioles, nNOS was found in epithelium at all levels including the alveolar wall. iNOS and nNOS were also detected in airway and vascular smooth muscle. The cellular distribution of all three isoforms was similar in fetal, newborn, and adult lungs. Findings in the epithelium were confirmed by isoform-specific reverse transcription- polymerase chain reaction assays and NADPH diaphorase histochemistry. Thus the three NOS isoforms are commonly expressed in proximal lung epithelium and are differentially expressed in distal lung epithelium. All three isoforms may be important sources of epithelium-derived NO throughout lung development.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume276
Issue number2 20-2
StatePublished - 1999

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Nitric Oxide Synthase
Protein Isoforms
Epithelium
Lung
Bronchioles
Nitric Oxide
Physiological Phenomena
NADPH Dehydrogenase
Respiratory Mucosa
Vascular Smooth Muscle
Reverse Transcription
Sheep
Immunohistochemistry
Mothers
Pregnancy
Polymerase Chain Reaction

Keywords

  • Airway
  • Inducible nitric oxide synthase
  • Neuronal nitric oxide synthase
  • Reduced nicotinamide adenine dinucleotide phosphate diaphorase
  • Smooth muscle

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Nitric oxide synthase isoform expression in the developing lung epithelium. / Sherman, T. S.; Chen, Z.; Yuhanna, I. S.; Lau, K. S.; Margraf, L. R.; Shaul, P. W.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 276, No. 2 20-2, 1999.

Research output: Contribution to journalArticle

Sherman, T. S. ; Chen, Z. ; Yuhanna, I. S. ; Lau, K. S. ; Margraf, L. R. ; Shaul, P. W. / Nitric oxide synthase isoform expression in the developing lung epithelium. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1999 ; Vol. 276, No. 2 20-2.
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