NMDA receptor-dependent long-term synaptic depression in the entorhinal cortex in vitro

Saïd Kourrich, C. Andrew Chapman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The entorhinal cortex receives a large projection from the piriform (primary olfactory) cortex and, in turn, provides the hippocampal formation with most of its cortical sensory input. Synaptic plasticity in this pathway may therefore affect the processing of olfactory information and memory encoding. We have recently found that long-term synaptic depression (LTD) can be induced in this pathway in vivo by repetitive paired-pulse stimulation but not by low-frequency (1 Hz) stimulation with single pulses. Here, we have used field potential recordings to investigate the stimulation parameters and transmitter receptors required for the induction of LTD in the rat entorhinal cortex in vitro. The effectiveness of low-frequency stimulation (900 pulses at 1 or 5 Hz) and repeated delivery of pairs of stimulation pulses (30-ms interpulse interval) was assessed. Only repeated paired-pulse stimulation resulted in lasting LTD, and a low-intensity paired-pulse stimulation protocol that induces LTD in vivo was only effective in the presence of the GABAA receptor antagonist bicuculline (50 μM). LTD could also be induced in normal ACSF, however, by increasing the number of pulse-pairs delivered and by increasing the stimulation intensity during LTD induction. The induction of LTD was blocked by constant bath application of the N-methyl-D-aspartate (NMDA) glutamate receptor antagonist D-2-amino-5-phosphonovalerate (50 μM), indicating that LTD is dependent on NMDA receptor activation. However, LTD was not blocked by the group I/II mGluR antagonist (RS)-α-ethyl-4-carboxyphenylglycine (500 μM) or by bicuculline (50 μM). The induction of LTD in the entorhinal cortex in vitro is therefore dependent on intense stimulation that recruits activation of NMDA receptors, but does not require concurrent activation of mGluRs or inhibitory synaptic inputs.

Original languageEnglish (US)
Pages (from-to)2112-2119
Number of pages8
JournalJournal of Neurophysiology
Volume89
Issue number4
DOIs
StatePublished - Apr 1 2003

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Long-Term Synaptic Depression
Entorhinal Cortex
N-Methyl-D-Aspartate Receptors
Bicuculline
In Vitro Techniques
2-Amino-5-phosphonovalerate
GABA-A Receptor Antagonists
Excitatory Amino Acid Antagonists
Neuronal Plasticity
Baths
Automatic Data Processing
Hippocampus

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

NMDA receptor-dependent long-term synaptic depression in the entorhinal cortex in vitro. / Kourrich, Saïd; Chapman, C. Andrew.

In: Journal of Neurophysiology, Vol. 89, No. 4, 01.04.2003, p. 2112-2119.

Research output: Contribution to journalArticle

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