No dosage effect of recombinational hotspots in the mouse major histocompatibility complex

Masayasu Yoshino, Tomoko Sagai, Kirsten Fischer Lindahl, Yutaka Toyoda, Toshihiko Shiroishi, Kazuo Moriwaki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The sites of meiotic recombination in the proximal region of the mouse major histocompatibility complex (MHC) are clustered at hotspots. Some MHC haplotypes derived from Asian wild mice increase the frequency of recombination at such hotspots when heterozygous with standard laboratory haplotypes. The wm7 and cas3 haplotypes, have a hotspot close to the Lmp-2 gene (Lmp-2 hotspot), and the cas4 haplotype has a hotspot about 100 kilobase (kb) proximal, close to the Pb gene (Pb hotspot). To examine the effect of a double dose of hotspots, we estimated the rate of recombination and determined the location of the breakpoints in crosses of wm7/cas3 and wm7/cas4. In 3570 backcross progeny we identified 29 new recombinants in the H-2K to Ab interval, at a frequency of 0.81%. This frequency is 40-fold higher than in crosses between laboratory haplotypes and very similar to those previously obtained in crosses between these wild and standard laboratory haplotypes. Thus, a double dose of hotspots has no additive effect on the frequency of meiotic recombination. The site-specificity of recombination was also conserved. Twenty-three breakpoints were confined within 5.4 kb in the Lmp-2 hotspot, and six breakpoints from the cas4 cross were located in the Pb hotspot, which we have now confined to a 15 kb segment.

Original languageEnglish (US)
Pages (from-to)381-389
Number of pages9
JournalImmunogenetics
Volume39
Issue number6
DOIs
StatePublished - Apr 1994

Fingerprint

Major Histocompatibility Complex
Haplotypes
Genetic Recombination
Genes

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Yoshino, M., Sagai, T., Fischer Lindahl, K., Toyoda, Y., Shiroishi, T., & Moriwaki, K. (1994). No dosage effect of recombinational hotspots in the mouse major histocompatibility complex. Immunogenetics, 39(6), 381-389. https://doi.org/10.1007/BF00176154

No dosage effect of recombinational hotspots in the mouse major histocompatibility complex. / Yoshino, Masayasu; Sagai, Tomoko; Fischer Lindahl, Kirsten; Toyoda, Yutaka; Shiroishi, Toshihiko; Moriwaki, Kazuo.

In: Immunogenetics, Vol. 39, No. 6, 04.1994, p. 381-389.

Research output: Contribution to journalArticle

Yoshino, M, Sagai, T, Fischer Lindahl, K, Toyoda, Y, Shiroishi, T & Moriwaki, K 1994, 'No dosage effect of recombinational hotspots in the mouse major histocompatibility complex', Immunogenetics, vol. 39, no. 6, pp. 381-389. https://doi.org/10.1007/BF00176154
Yoshino M, Sagai T, Fischer Lindahl K, Toyoda Y, Shiroishi T, Moriwaki K. No dosage effect of recombinational hotspots in the mouse major histocompatibility complex. Immunogenetics. 1994 Apr;39(6):381-389. https://doi.org/10.1007/BF00176154
Yoshino, Masayasu ; Sagai, Tomoko ; Fischer Lindahl, Kirsten ; Toyoda, Yutaka ; Shiroishi, Toshihiko ; Moriwaki, Kazuo. / No dosage effect of recombinational hotspots in the mouse major histocompatibility complex. In: Immunogenetics. 1994 ; Vol. 39, No. 6. pp. 381-389.
@article{0fd059827704439cbe0ac55a101aded8,
title = "No dosage effect of recombinational hotspots in the mouse major histocompatibility complex",
abstract = "The sites of meiotic recombination in the proximal region of the mouse major histocompatibility complex (MHC) are clustered at hotspots. Some MHC haplotypes derived from Asian wild mice increase the frequency of recombination at such hotspots when heterozygous with standard laboratory haplotypes. The wm7 and cas3 haplotypes, have a hotspot close to the Lmp-2 gene (Lmp-2 hotspot), and the cas4 haplotype has a hotspot about 100 kilobase (kb) proximal, close to the Pb gene (Pb hotspot). To examine the effect of a double dose of hotspots, we estimated the rate of recombination and determined the location of the breakpoints in crosses of wm7/cas3 and wm7/cas4. In 3570 backcross progeny we identified 29 new recombinants in the H-2K to Ab interval, at a frequency of 0.81{\%}. This frequency is 40-fold higher than in crosses between laboratory haplotypes and very similar to those previously obtained in crosses between these wild and standard laboratory haplotypes. Thus, a double dose of hotspots has no additive effect on the frequency of meiotic recombination. The site-specificity of recombination was also conserved. Twenty-three breakpoints were confined within 5.4 kb in the Lmp-2 hotspot, and six breakpoints from the cas4 cross were located in the Pb hotspot, which we have now confined to a 15 kb segment.",
author = "Masayasu Yoshino and Tomoko Sagai and {Fischer Lindahl}, Kirsten and Yutaka Toyoda and Toshihiko Shiroishi and Kazuo Moriwaki",
year = "1994",
month = "4",
doi = "10.1007/BF00176154",
language = "English (US)",
volume = "39",
pages = "381--389",
journal = "Immunogenetics",
issn = "0093-7711",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - No dosage effect of recombinational hotspots in the mouse major histocompatibility complex

AU - Yoshino, Masayasu

AU - Sagai, Tomoko

AU - Fischer Lindahl, Kirsten

AU - Toyoda, Yutaka

AU - Shiroishi, Toshihiko

AU - Moriwaki, Kazuo

PY - 1994/4

Y1 - 1994/4

N2 - The sites of meiotic recombination in the proximal region of the mouse major histocompatibility complex (MHC) are clustered at hotspots. Some MHC haplotypes derived from Asian wild mice increase the frequency of recombination at such hotspots when heterozygous with standard laboratory haplotypes. The wm7 and cas3 haplotypes, have a hotspot close to the Lmp-2 gene (Lmp-2 hotspot), and the cas4 haplotype has a hotspot about 100 kilobase (kb) proximal, close to the Pb gene (Pb hotspot). To examine the effect of a double dose of hotspots, we estimated the rate of recombination and determined the location of the breakpoints in crosses of wm7/cas3 and wm7/cas4. In 3570 backcross progeny we identified 29 new recombinants in the H-2K to Ab interval, at a frequency of 0.81%. This frequency is 40-fold higher than in crosses between laboratory haplotypes and very similar to those previously obtained in crosses between these wild and standard laboratory haplotypes. Thus, a double dose of hotspots has no additive effect on the frequency of meiotic recombination. The site-specificity of recombination was also conserved. Twenty-three breakpoints were confined within 5.4 kb in the Lmp-2 hotspot, and six breakpoints from the cas4 cross were located in the Pb hotspot, which we have now confined to a 15 kb segment.

AB - The sites of meiotic recombination in the proximal region of the mouse major histocompatibility complex (MHC) are clustered at hotspots. Some MHC haplotypes derived from Asian wild mice increase the frequency of recombination at such hotspots when heterozygous with standard laboratory haplotypes. The wm7 and cas3 haplotypes, have a hotspot close to the Lmp-2 gene (Lmp-2 hotspot), and the cas4 haplotype has a hotspot about 100 kilobase (kb) proximal, close to the Pb gene (Pb hotspot). To examine the effect of a double dose of hotspots, we estimated the rate of recombination and determined the location of the breakpoints in crosses of wm7/cas3 and wm7/cas4. In 3570 backcross progeny we identified 29 new recombinants in the H-2K to Ab interval, at a frequency of 0.81%. This frequency is 40-fold higher than in crosses between laboratory haplotypes and very similar to those previously obtained in crosses between these wild and standard laboratory haplotypes. Thus, a double dose of hotspots has no additive effect on the frequency of meiotic recombination. The site-specificity of recombination was also conserved. Twenty-three breakpoints were confined within 5.4 kb in the Lmp-2 hotspot, and six breakpoints from the cas4 cross were located in the Pb hotspot, which we have now confined to a 15 kb segment.

UR - http://www.scopus.com/inward/record.url?scp=0028267799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028267799&partnerID=8YFLogxK

U2 - 10.1007/BF00176154

DO - 10.1007/BF00176154

M3 - Article

C2 - 7910587

AN - SCOPUS:0028267799

VL - 39

SP - 381

EP - 389

JO - Immunogenetics

JF - Immunogenetics

SN - 0093-7711

IS - 6

ER -