TY - JOUR
T1 - Norepinephrine inhibits gamma-interferon-induced MHC class II (Ia) antigen expression on cultured brain astrocytes
AU - Frohman, Elliot
AU - Vayuvegula, Bharathi
AU - van den Noort, Stanley
AU - Gupta, Sudhir
N1 - Funding Information:
The authors wish to thank Mrs. Allison McCormick for her invaluablec ontri-bution in the establishmenotf the astrocytec ultures.I n additionw e wish to thank C.N. Honda for his help in the preparationo f the manuscriptT. his work was supportedi n part by a grant from USPHS AG 04362 and by NS 21377 to E.G. Jones from the National Instituteso f Health, U.S. Public Health Service.
PY - 1988/1
Y1 - 1988/1
N2 - Recent evidence that astrocytes can be induced to express the class II major histocompatability (MHC) antigens suggests that these cells may be involved in the development of intracerebral immune responses. The principal inducer of MHC class II antigen (Ia) expression is a soluble lymphokine, gamma-interferon (γ-IFN). Normally astrocytes do not express significant levels of Ia antigens despite the fact that agents such as γ-IFN may be present in the central nervous system (CNS). Here we report that a major neurotransmitter, norepinephrine (NE), inhibits, in a dose-response fashion, the ability of γ-IFN to induce Ia antigen expression on cultured astrocytes derived from newborn BALB/c mice. This finding may indicate that the brain contains inhibitory modulators that serve to prevent the up-regulation of intracerebral immune responsiveness.
AB - Recent evidence that astrocytes can be induced to express the class II major histocompatability (MHC) antigens suggests that these cells may be involved in the development of intracerebral immune responses. The principal inducer of MHC class II antigen (Ia) expression is a soluble lymphokine, gamma-interferon (γ-IFN). Normally astrocytes do not express significant levels of Ia antigens despite the fact that agents such as γ-IFN may be present in the central nervous system (CNS). Here we report that a major neurotransmitter, norepinephrine (NE), inhibits, in a dose-response fashion, the ability of γ-IFN to induce Ia antigen expression on cultured astrocytes derived from newborn BALB/c mice. This finding may indicate that the brain contains inhibitory modulators that serve to prevent the up-regulation of intracerebral immune responsiveness.
KW - Astrocyte
KW - Flow cytometry
KW - Ia antigen expression
KW - Immunofluorescence
KW - Interferon-γ
KW - Norepinephrine
UR - http://www.scopus.com/inward/record.url?scp=0023700535&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023700535&partnerID=8YFLogxK
U2 - 10.1016/0165-5728(88)90017-3
DO - 10.1016/0165-5728(88)90017-3
M3 - Article
C2 - 2826540
AN - SCOPUS:0023700535
SN - 0165-5728
VL - 17
SP - 89
EP - 101
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 2
ER -