Novel HY peptide antigens presented by HLA-B271

William A. Simmons, Scott G. Summerfield, Derry C. Roopenian, Clive A. Slaughter, Aamir R. Zuberi, Simon J. Gaskell, Robert S. Bordoli, John Hoyes, Carolyn R. Moomaw, Robert A. Colbert, Louise Y W Leong, Geoffrey W. Butcher, Robert E Hammer, Joel D Taurog

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have identified two peptides corresponding to the male-specific HY minor histocompatibility Ags presented by HLA-B27 in transgenic rodents, isolated from whole cell extracts and from immunoprecipitated B27 molecules of male B27 rat spleen cells. HPLC peptide fractions that sensitized female B27 targets for lysis by B27-restricted anti-HY CTL were analyzed by electrospray tandem mass spectrometry using a new highly sensitive quadrupole/time-of-flight instrument. Two peptide sequences were obtained, KQYQKSTER and AVLNKSNREVR. Synthetic peptides corresponding to these sequences bound B27 in vitro and were recognized by distinct B27-restricted anti-HY CTL populations. Neither peptide sequence entirely matches known protein sequences or shows a resemblance to known Y chromosome genes, but both show homology to known autosomally encoded proteins. Both peptides were shown to be controlled by the Sxr6 segment of the short arm of the mouse Y chromosome, a segment known to contain all previously identified HY Ags. Taken together, these findings suggest that the two peptides arise as a result of Y chromosome-regulated control of one or more autosomal gene products. Although arginine at position 2 is a dominant anchor residue for peptides bound to B27, neither B27-presented HY sequence contains this residue. These studies, employing sensitive new methodology for identification of MHC-bound peptides, significantly extend the complexity of the genetic basis of HY Acs and expand the repertoire of anlisenicallv active peptides bound to B27.

Original languageEnglish (US)
Pages (from-to)2750-2759
Number of pages10
JournalJournal of Immunology
Volume159
Issue number6
StatePublished - 1997

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H-Y Antigen
Peptides
Y Chromosome
Y-Linked Genes
HLA-B27 Antigen
Histocompatibility
Tandem Mass Spectrometry
Cell Extracts
Arginine
Rodentia
Proteins
Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

Simmons, W. A., Summerfield, S. G., Roopenian, D. C., Slaughter, C. A., Zuberi, A. R., Gaskell, S. J., ... Taurog, J. D. (1997). Novel HY peptide antigens presented by HLA-B271. Journal of Immunology, 159(6), 2750-2759.

Novel HY peptide antigens presented by HLA-B271. / Simmons, William A.; Summerfield, Scott G.; Roopenian, Derry C.; Slaughter, Clive A.; Zuberi, Aamir R.; Gaskell, Simon J.; Bordoli, Robert S.; Hoyes, John; Moomaw, Carolyn R.; Colbert, Robert A.; Leong, Louise Y W; Butcher, Geoffrey W.; Hammer, Robert E; Taurog, Joel D.

In: Journal of Immunology, Vol. 159, No. 6, 1997, p. 2750-2759.

Research output: Contribution to journalArticle

Simmons, WA, Summerfield, SG, Roopenian, DC, Slaughter, CA, Zuberi, AR, Gaskell, SJ, Bordoli, RS, Hoyes, J, Moomaw, CR, Colbert, RA, Leong, LYW, Butcher, GW, Hammer, RE & Taurog, JD 1997, 'Novel HY peptide antigens presented by HLA-B271', Journal of Immunology, vol. 159, no. 6, pp. 2750-2759.
Simmons WA, Summerfield SG, Roopenian DC, Slaughter CA, Zuberi AR, Gaskell SJ et al. Novel HY peptide antigens presented by HLA-B271. Journal of Immunology. 1997;159(6):2750-2759.
Simmons, William A. ; Summerfield, Scott G. ; Roopenian, Derry C. ; Slaughter, Clive A. ; Zuberi, Aamir R. ; Gaskell, Simon J. ; Bordoli, Robert S. ; Hoyes, John ; Moomaw, Carolyn R. ; Colbert, Robert A. ; Leong, Louise Y W ; Butcher, Geoffrey W. ; Hammer, Robert E ; Taurog, Joel D. / Novel HY peptide antigens presented by HLA-B271. In: Journal of Immunology. 1997 ; Vol. 159, No. 6. pp. 2750-2759.
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abstract = "We have identified two peptides corresponding to the male-specific HY minor histocompatibility Ags presented by HLA-B27 in transgenic rodents, isolated from whole cell extracts and from immunoprecipitated B27 molecules of male B27 rat spleen cells. HPLC peptide fractions that sensitized female B27 targets for lysis by B27-restricted anti-HY CTL were analyzed by electrospray tandem mass spectrometry using a new highly sensitive quadrupole/time-of-flight instrument. Two peptide sequences were obtained, KQYQKSTER and AVLNKSNREVR. Synthetic peptides corresponding to these sequences bound B27 in vitro and were recognized by distinct B27-restricted anti-HY CTL populations. Neither peptide sequence entirely matches known protein sequences or shows a resemblance to known Y chromosome genes, but both show homology to known autosomally encoded proteins. Both peptides were shown to be controlled by the Sxr6 segment of the short arm of the mouse Y chromosome, a segment known to contain all previously identified HY Ags. Taken together, these findings suggest that the two peptides arise as a result of Y chromosome-regulated control of one or more autosomal gene products. Although arginine at position 2 is a dominant anchor residue for peptides bound to B27, neither B27-presented HY sequence contains this residue. These studies, employing sensitive new methodology for identification of MHC-bound peptides, significantly extend the complexity of the genetic basis of HY Acs and expand the repertoire of anlisenicallv active peptides bound to B27.",
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AU - Simmons, William A.

AU - Summerfield, Scott G.

AU - Roopenian, Derry C.

AU - Slaughter, Clive A.

AU - Zuberi, Aamir R.

AU - Gaskell, Simon J.

AU - Bordoli, Robert S.

AU - Hoyes, John

AU - Moomaw, Carolyn R.

AU - Colbert, Robert A.

AU - Leong, Louise Y W

AU - Butcher, Geoffrey W.

AU - Hammer, Robert E

AU - Taurog, Joel D

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