Novel insecticidal toxins from the venom of the spider Segestria florentina

A. Lipkin, S. Kozlov, E. Nosyreva, A. Blake, J. D. Windass, E. Grishin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Three insecticidal polypeptide toxins (F5.5, F5.6, F5.7) with molecular masses 4973, 4993 and 5159 Da were isolated from the venom of the central Asian spider Segestria florentina. These toxins caused the complete flaccid paralysis of Heliothis virescens (Lepidoptera: Noctuidae) larvae (LD50 4-10 μg/g), whereas they were inactive upon intravenous injections into mice. On the basis of N-terminal amino acid sequences a family of eight genes encoding highly homologues polypeptides (SFI1-SFI8) was revealed, some of which encode polypeptides actually demonstrated to be present in S. florentina venom. All deduced polypeptides consist of 46 amino acids residues. Comparison of primary structures of SFI1-SFI8 with other spider toxins suggests that this family might share structural and functional relationships with other small spider neurotoxins, several of which are known to be highly selective agonists/antagonists of different voltage-dependent Ca2+ channels.

Original languageEnglish (US)
Pages (from-to)125-130
Number of pages6
JournalToxicon
Volume40
Issue number2
DOIs
StatePublished - 2002

Fingerprint

Spider Venoms
Spiders
Peptides
Venoms
Amino Acids
Lepidoptera
Gene encoding
Lethal Dose 50
Neurotoxins
Molecular mass
Intravenous Injections
Paralysis
Larva
Amino Acid Sequence
Electric potential
Genes

Keywords

  • Flaccid paralysis
  • Insecticidal polypeptide toxins
  • Segstria florentina
  • Spider venom

ASJC Scopus subject areas

  • Toxicology

Cite this

Lipkin, A., Kozlov, S., Nosyreva, E., Blake, A., Windass, J. D., & Grishin, E. (2002). Novel insecticidal toxins from the venom of the spider Segestria florentina. Toxicon, 40(2), 125-130. https://doi.org/10.1016/S0041-0101(01)00181-7

Novel insecticidal toxins from the venom of the spider Segestria florentina. / Lipkin, A.; Kozlov, S.; Nosyreva, E.; Blake, A.; Windass, J. D.; Grishin, E.

In: Toxicon, Vol. 40, No. 2, 2002, p. 125-130.

Research output: Contribution to journalArticle

Lipkin, A, Kozlov, S, Nosyreva, E, Blake, A, Windass, JD & Grishin, E 2002, 'Novel insecticidal toxins from the venom of the spider Segestria florentina', Toxicon, vol. 40, no. 2, pp. 125-130. https://doi.org/10.1016/S0041-0101(01)00181-7
Lipkin A, Kozlov S, Nosyreva E, Blake A, Windass JD, Grishin E. Novel insecticidal toxins from the venom of the spider Segestria florentina. Toxicon. 2002;40(2):125-130. https://doi.org/10.1016/S0041-0101(01)00181-7
Lipkin, A. ; Kozlov, S. ; Nosyreva, E. ; Blake, A. ; Windass, J. D. ; Grishin, E. / Novel insecticidal toxins from the venom of the spider Segestria florentina. In: Toxicon. 2002 ; Vol. 40, No. 2. pp. 125-130.
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