Objective evaluation of the microscopic image sharpness for diagnostic metaphase chromosomes

Yuchen Qiu, Yuhua Li, Liqiang Ren, Wei R. Chen, Bin Zheng, Shibo Li, Hong Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


The purpose of this study is to objectively investigate image sharpness of the metaphase chromosomes at different scanning speeds. In cytogenetic imaging, high scanning speed is sometimes applied to optimize the efficiency. However, at high scanning speed, the obtained image may be deteriorated, due to the scanning blur, under exposure and random vibration of the scanning stage. In this study, the image sharpness of metaphase chromosomes is objectively evaluated. A standard resolution target and several metaphase chromosomes are first imaged under different speeds, by a prototype scanning microscope. Then the sharpness of the acquired images is objectively assessed using a sharpness function. For this prototype system, the results demonstrate that the image sharpness of the metaphase chromosomes is optimized when the scanning speed is 0.8mm/s, under the specified experimental conditions. The results of this study may be useful for optimizing the efficiency and quality of clinical cytogenetic imaging procedures.

Original languageEnglish (US)
Title of host publicationBiophotonics and Immune Responses VIII
StatePublished - 2013
Externally publishedYes
EventBiophotonics and Immune Responses VIII - San Francisco, CA, United States
Duration: Feb 4 2013Feb 4 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceBiophotonics and Immune Responses VIII
Country/TerritoryUnited States
CitySan Francisco, CA


  • cytogenetic imaging
  • image sharpness
  • objective evaluation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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