The enzyme adenylate cyclase is critically involved in the regulation of vasodilatation in the developing pulmonary circulation in that it mediates the vascular smooth muscle effects of β-adrenergic agonists and vaso-dilatory prostaglandins. These agonists activate receptors coupled to the catalytic subunit of the enzyme by stimulatory guanine nucleotide-dependent regulatory proteins. We examined the ontogeny of the function of the adenylate cyclase system in intrapulmonary arterial segments from fetal lambs at 110-115 (Fl) and 125-135 d gestation (F2) and from postnatal lambs at 7-14 (PI) and 35-56 d of age (P2). The function of the intact enzyme system and its components was assessed in incubations measuring cAMP accumulation during phosphodiesterase inhibition. β-Ad-renergic mediation of adenylate cyclase was examined because the binding characteristics of the smooth muscle receptors can be readily quantified. Nonstimulated (basal) cAMP accumulation was similar in Fl and F2, it increased 8-fold from F2 to PI, and it was equivalent in PI and P2. cAMP accumulation with isoproterenol increased 5.9-fold from Fl to F2 and was similar in F2, PI, and P2. Radioligand binding studies revealed that the greater response to isoproterenol in F2 versus Fl is not related to an increase in β-adrenergic receptor density or affinity. cAMP accumulation with forskolin, which activates adenylate cyclase by actions that involve both the stimulatory guanine nucleotide-dependent regulatory protein and the catalytic sub-unit, was similar in the four age groups, indicating that there are no maturational changes in the function of these enzyme system components. As such, the enhancement in β-adrenergic mediation of the enzyme during the 3rd trimester may be related to changes in receptor-guaninne nucleotide-dependent regulatory protein coupling. It is concluded that there are marked developmental alterations in the function of the adenylate cyclase system in the intrapulmonary arteries during late gestation that may serve to optimize the capacity of this mediator of pulmonary vasodilatation in the perinatal period.
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health