Orexins acting at native OX1 receptor in colon cancer and neuroblastoma cells or at recombinant OX1 receptor suppress cell growth by inducing apoptosis

Patricia Rouet-Benzineb, Christiane Rouyer-Fessard, Anne Jarry, Virgile Avondo, Cécile Pouzet, Masashi Yanagisawa, Christian Laboisse, Marc Laburthe, Thierry Voisin

Research output: Contribution to journalArticle

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Abstract

Screening of 26 gut peptides for their ability to inhibit growth of human colon cancer HT29-D4 cells grown in 10% fetal calf serum identified orexin-A and orexin-B as anti-growth factors. Upon addition of either orexin (1 μM), suppression of cell growth was total after 24 h and >70% after 48 or 72 h, with an EC50 of 5 nM peptide. Orexins did not alter proliferation but promoted apoptosis as demonstrated by morphological changes in cell shape, DNA fragmentation, chromatin condensation, cytochrome c release into cytosol, and activation of caspase-3 and caspase-7. The serpentine G protein-coupled orexin receptor OX1R but not OX2R was expressed in HT29-D4 cells and mediated orexin-induced Ca2+ transients in HT29-D4 cells. The expression of OX1R and the pro-apoptotic effects of orexins were also indicated in other colon cancer cell lines including Caco-2, SW480, and LoVo but, most interestingly, not in normal colonic epithelial cells. The role of OX1R in mediating apoptosis was further demonstrated by transfecting Chinese hamster ovary cells with OX1R cDNA, which conferred the ability of orexins to promote apoptosis. A neuroblastoma cell line SK-N-MC, which expresses OX1R, also underwent growth suppression and apoptosis upon treatment with orexins. Promotion of apoptosis appears to be an intrinsic property of OX1R regardless of the cell type where it is expressed. In conclusion, orexins, acting at native or recombinant OX1R, are pro-apoptotic peptides. These findings add a new dimension to the biological activities of these neuropeptides, which may have important implications in health and disease, in particular colon cancer.

Original languageEnglish (US)
Pages (from-to)45875-45886
Number of pages12
JournalJournal of Biological Chemistry
Volume279
Issue number44
DOIs
StatePublished - Oct 29 2004

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Orexin Receptors
Cell growth
Neuroblastoma
Colonic Neoplasms
Apoptosis
HT29 Cells
Growth
Cells
Peptides
Caspase 7
Cell Line
Cell Shape
DNA Fragmentation
G-Protein-Coupled Receptors
Cricetulus
Cytochromes c
Bioactivity
Orexins
Neuropeptides
GTP-Binding Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Orexins acting at native OX1 receptor in colon cancer and neuroblastoma cells or at recombinant OX1 receptor suppress cell growth by inducing apoptosis. / Rouet-Benzineb, Patricia; Rouyer-Fessard, Christiane; Jarry, Anne; Avondo, Virgile; Pouzet, Cécile; Yanagisawa, Masashi; Laboisse, Christian; Laburthe, Marc; Voisin, Thierry.

In: Journal of Biological Chemistry, Vol. 279, No. 44, 29.10.2004, p. 45875-45886.

Research output: Contribution to journalArticle

Rouet-Benzineb, Patricia ; Rouyer-Fessard, Christiane ; Jarry, Anne ; Avondo, Virgile ; Pouzet, Cécile ; Yanagisawa, Masashi ; Laboisse, Christian ; Laburthe, Marc ; Voisin, Thierry. / Orexins acting at native OX1 receptor in colon cancer and neuroblastoma cells or at recombinant OX1 receptor suppress cell growth by inducing apoptosis. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 44. pp. 45875-45886.
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T1 - Orexins acting at native OX1 receptor in colon cancer and neuroblastoma cells or at recombinant OX1 receptor suppress cell growth by inducing apoptosis

AU - Rouet-Benzineb, Patricia

AU - Rouyer-Fessard, Christiane

AU - Jarry, Anne

AU - Avondo, Virgile

AU - Pouzet, Cécile

AU - Yanagisawa, Masashi

AU - Laboisse, Christian

AU - Laburthe, Marc

AU - Voisin, Thierry

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Y1 - 2004/10/29

N2 - Screening of 26 gut peptides for their ability to inhibit growth of human colon cancer HT29-D4 cells grown in 10% fetal calf serum identified orexin-A and orexin-B as anti-growth factors. Upon addition of either orexin (1 μM), suppression of cell growth was total after 24 h and >70% after 48 or 72 h, with an EC50 of 5 nM peptide. Orexins did not alter proliferation but promoted apoptosis as demonstrated by morphological changes in cell shape, DNA fragmentation, chromatin condensation, cytochrome c release into cytosol, and activation of caspase-3 and caspase-7. The serpentine G protein-coupled orexin receptor OX1R but not OX2R was expressed in HT29-D4 cells and mediated orexin-induced Ca2+ transients in HT29-D4 cells. The expression of OX1R and the pro-apoptotic effects of orexins were also indicated in other colon cancer cell lines including Caco-2, SW480, and LoVo but, most interestingly, not in normal colonic epithelial cells. The role of OX1R in mediating apoptosis was further demonstrated by transfecting Chinese hamster ovary cells with OX1R cDNA, which conferred the ability of orexins to promote apoptosis. A neuroblastoma cell line SK-N-MC, which expresses OX1R, also underwent growth suppression and apoptosis upon treatment with orexins. Promotion of apoptosis appears to be an intrinsic property of OX1R regardless of the cell type where it is expressed. In conclusion, orexins, acting at native or recombinant OX1R, are pro-apoptotic peptides. These findings add a new dimension to the biological activities of these neuropeptides, which may have important implications in health and disease, in particular colon cancer.

AB - Screening of 26 gut peptides for their ability to inhibit growth of human colon cancer HT29-D4 cells grown in 10% fetal calf serum identified orexin-A and orexin-B as anti-growth factors. Upon addition of either orexin (1 μM), suppression of cell growth was total after 24 h and >70% after 48 or 72 h, with an EC50 of 5 nM peptide. Orexins did not alter proliferation but promoted apoptosis as demonstrated by morphological changes in cell shape, DNA fragmentation, chromatin condensation, cytochrome c release into cytosol, and activation of caspase-3 and caspase-7. The serpentine G protein-coupled orexin receptor OX1R but not OX2R was expressed in HT29-D4 cells and mediated orexin-induced Ca2+ transients in HT29-D4 cells. The expression of OX1R and the pro-apoptotic effects of orexins were also indicated in other colon cancer cell lines including Caco-2, SW480, and LoVo but, most interestingly, not in normal colonic epithelial cells. The role of OX1R in mediating apoptosis was further demonstrated by transfecting Chinese hamster ovary cells with OX1R cDNA, which conferred the ability of orexins to promote apoptosis. A neuroblastoma cell line SK-N-MC, which expresses OX1R, also underwent growth suppression and apoptosis upon treatment with orexins. Promotion of apoptosis appears to be an intrinsic property of OX1R regardless of the cell type where it is expressed. In conclusion, orexins, acting at native or recombinant OX1R, are pro-apoptotic peptides. These findings add a new dimension to the biological activities of these neuropeptides, which may have important implications in health and disease, in particular colon cancer.

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