Orthotopic hind-limb transplantation in rats

Robert Sucher, Rupert Oberhuber, Christian Margreiter, Guido Rumberg, Rishi Jindal, W. P. Andrew Lee, Raimund Margreiter, Johann Pratschke, Stefan Schneeberger, Gerald Brandacher

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Composite tissue allotransplantation (CTA) now represents a valid therapeutic option after the loss of a hand, forearm or digits and has become a novel therapeutic entity in reconstructive surgery. However, long term high-dose multi-drug immunosuppressive therapy is required to ensure graft survival, bearing the risk of serious side effects which halters broader application. Further progression in this field may depend on better understanding of basic immunology and ischemia reperfusion injury in composite tissue grafts. To date, orthotopic hind limb transplantation in rats has been the preferred rodent model for reconstructive transplantation (RT), however, it is an extremely demanding procedure that requires extraordinary microsurgical skills for reattachment of vasculature, bones, muscles and nerves. We have introduced the vascular cuff anastomosis technique to this model, providing a rapid and reliable approach to rat hind limb transplantation. This technique simplifies and shortens the surgical procedure and enables surgeons with basic microsurgical experience to successfully perform the operation with high survival and low complication rates. The technique seems to be well suited for immunological as well as ischemia reperfusion injury (IRI) studies.

Original languageEnglish (US)
Article numbere2022
JournalJournal of Visualized Experiments
Issue number41
DOIs
StatePublished - Jul 2010
Externally publishedYes

Keywords

  • Composite tissue
  • Hind limb
  • Issue 41
  • JoVE Immunology
  • Rat
  • Reconstructive transplantation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Sucher, R., Oberhuber, R., Margreiter, C., Rumberg, G., Jindal, R., Andrew Lee, W. P., Margreiter, R., Pratschke, J., Schneeberger, S., & Brandacher, G. (2010). Orthotopic hind-limb transplantation in rats. Journal of Visualized Experiments, (41), [e2022]. https://doi.org/10.3791/2022