Overexpression of sigma-1 receptor inhibits ADAM10 and ADAM17 mediated shedding in vitro

Juan Li, Bin Liu, Xiaofei Gao, Zhixing Ma, Tianyi CaoSong, Yan ai Mei, Yufang Zheng

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The sigma-1 receptor is a molecular chaperone protein highly enriched in the brain. Recent studies linked it to many diseases, such as drug addition, Alzheimer's disease, stroke, depression, and even cancer. Sigma-1 receptor is enriched in lipid rafts, which are membrane microdomains essential in signaling processes. One of those signaling processes is ADAM17- and ADAM10-dependent ectodomain shedding. By using an alkaline phosphatase tagged substrate reporter system, we have shown that ADAM10-dependent BTC shedding was very sensitive to both membrane lipid component change and sigma-1 receptor agonist DHEAS treatment while ADAM17-dependent HB-EGF shedding was not; and overexpression of sigma-1 receptor diminished ADAM17- and ADAM10-dependent shedding. Our results indicate that sigma-1 receptor plays an important role in modifying the function of transmembrane proteases.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalProtein and Cell
Volume3
Issue number2
DOIs
StatePublished - Feb 2012

Keywords

  • ADAM10
  • ADAM17
  • lipid raft
  • shedding
  • sigma-1 receptor

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Drug Discovery
  • Cell Biology

Fingerprint Dive into the research topics of 'Overexpression of sigma-1 receptor inhibits ADAM10 and ADAM17 mediated shedding in vitro'. Together they form a unique fingerprint.

Cite this