Abstract
Recent tools addressed to accelerate the different steps of the sample treatment for protein identification in modern workflows are reviewed and critically commented in this manuscript. Heating, microspin columns, ultrasonic energy, high pressure, infrared energy, microwave energy, alternating electric fields and microreactors are outlined as useful tools that can be used to accelerate all or some of the following steps for in-gel or in-liquid based approaches for protein identification: (i) protein dissolution/denaturation, (ii) protein reduction, (iii) protein alkylation and (iv) protein digestion. The advantages and drawbacks, along with the main differences among the different tools are also commented. Future prospects for hyphenation of methods are also discussed. Researchers are informed also in this work regarding the main problems to be found when implementing any of the above mentioned methods.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 151-159 |
| Number of pages | 9 |
| Journal | Analytica Chimica Acta |
| Volume | 650 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 21 2009 |
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Keywords
- Fast methods
- Mass spectrometry
- Protein identification
- Proteomics
ASJC Scopus subject areas
- Analytical Chemistry
- Environmental Chemistry
- Biochemistry
- Spectroscopy
Cite this
Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques. / Capelo, J. L.; Carreira, R.; Diniz, M.; Fernandes, L.; Galesio, M.; Lodeiro, C.; Santos, H. M.; Vale, G.
In: Analytica Chimica Acta, Vol. 650, No. 2, 21.09.2009, p. 151-159.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques
AU - Capelo, J. L.
AU - Carreira, R.
AU - Diniz, M.
AU - Fernandes, L.
AU - Galesio, M.
AU - Lodeiro, C.
AU - Santos, H. M.
AU - Vale, G.
PY - 2009/9/21
Y1 - 2009/9/21
N2 - Recent tools addressed to accelerate the different steps of the sample treatment for protein identification in modern workflows are reviewed and critically commented in this manuscript. Heating, microspin columns, ultrasonic energy, high pressure, infrared energy, microwave energy, alternating electric fields and microreactors are outlined as useful tools that can be used to accelerate all or some of the following steps for in-gel or in-liquid based approaches for protein identification: (i) protein dissolution/denaturation, (ii) protein reduction, (iii) protein alkylation and (iv) protein digestion. The advantages and drawbacks, along with the main differences among the different tools are also commented. Future prospects for hyphenation of methods are also discussed. Researchers are informed also in this work regarding the main problems to be found when implementing any of the above mentioned methods.
AB - Recent tools addressed to accelerate the different steps of the sample treatment for protein identification in modern workflows are reviewed and critically commented in this manuscript. Heating, microspin columns, ultrasonic energy, high pressure, infrared energy, microwave energy, alternating electric fields and microreactors are outlined as useful tools that can be used to accelerate all or some of the following steps for in-gel or in-liquid based approaches for protein identification: (i) protein dissolution/denaturation, (ii) protein reduction, (iii) protein alkylation and (iv) protein digestion. The advantages and drawbacks, along with the main differences among the different tools are also commented. Future prospects for hyphenation of methods are also discussed. Researchers are informed also in this work regarding the main problems to be found when implementing any of the above mentioned methods.
KW - Fast methods
KW - Mass spectrometry
KW - Protein identification
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=69249125736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=69249125736&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2009.07.034
DO - 10.1016/j.aca.2009.07.034
M3 - Review article
C2 - 19720186
AN - SCOPUS:69249125736
VL - 650
SP - 151
EP - 159
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 2
ER -