Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques

J. L. Capelo, R. Carreira, M. Diniz, L. Fernandes, M. Galesio, C. Lodeiro, H. M. Santos, G. Vale

Research output: Contribution to journalReview article

72 Citations (Scopus)

Abstract

Recent tools addressed to accelerate the different steps of the sample treatment for protein identification in modern workflows are reviewed and critically commented in this manuscript. Heating, microspin columns, ultrasonic energy, high pressure, infrared energy, microwave energy, alternating electric fields and microreactors are outlined as useful tools that can be used to accelerate all or some of the following steps for in-gel or in-liquid based approaches for protein identification: (i) protein dissolution/denaturation, (ii) protein reduction, (iii) protein alkylation and (iv) protein digestion. The advantages and drawbacks, along with the main differences among the different tools are also commented. Future prospects for hyphenation of methods are also discussed. Researchers are informed also in this work regarding the main problems to be found when implementing any of the above mentioned methods.

Original languageEnglish (US)
Pages (from-to)151-159
Number of pages9
JournalAnalytica Chimica Acta
Volume650
Issue number2
DOIs
StatePublished - Sep 21 2009

Fingerprint

Workflow
cleavage
Mass spectrometry
Mass Spectrometry
mass spectrometry
protein
Proteins
Protein Denaturation
Alkylation
Microwaves
Ultrasonics
Heating
Proteolysis
energy
Denaturation
Gels
future prospect
Research Personnel
Pressure
speed

Keywords

  • Fast methods
  • Mass spectrometry
  • Protein identification
  • Proteomics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Biochemistry
  • Spectroscopy

Cite this

Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques. / Capelo, J. L.; Carreira, R.; Diniz, M.; Fernandes, L.; Galesio, M.; Lodeiro, C.; Santos, H. M.; Vale, G.

In: Analytica Chimica Acta, Vol. 650, No. 2, 21.09.2009, p. 151-159.

Research output: Contribution to journalReview article

Capelo, J. L. ; Carreira, R. ; Diniz, M. ; Fernandes, L. ; Galesio, M. ; Lodeiro, C. ; Santos, H. M. ; Vale, G. / Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques. In: Analytica Chimica Acta. 2009 ; Vol. 650, No. 2. pp. 151-159.
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