Oxidation of lactate and acetate in rat skeletal muscle

Analysis by 13C-nuclear magnetic resonance spectroscopy

Loren A. Bertocci, John G. Jones, Craig R. Malloy, Ronald G. Victor, Gail D. Thomas

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The balance between carbohydrate and fatty acid utilization in skeletal muscle previously has been studied in vivo by using a variety of methods such as arteriovenous concentration differences and radioactive isotope tracer techniques. However, these methodologies provide only indirect estimates of substrate oxidation. We used 13C-nuclear magnetic resonance (NMR) spectroscopy and non-steady-state isotopomer analysis to directly quantify the relative oxidation of two competing exogenous substrates in rat skeletal muscles. We infused [1,2-13C]acetate and [3-13C]lactate intravenously in anesthetized rats during the final 30 min of 35 (n = 10) or 95 (n = 10) min of intense, unilateral, rhythmic hindlimb contractions. 13C-NMR spectroscopy and isotopomer analysis were performed on extracts of gastrocnemius and soleus muscles from both the contracting and contralateral resting hindlimbs. We found that 1) [13C]lactate and [13C]acetate were taken up and oxidized by both resting and contracting skeletal muscles; and 2) high-intensity muscle contractions altered the pattern of substrate utilization such that the relative oxidation of acetate decreased while that of lactate remained unchanged or increased. Based on these findings, we propose that 13C-NMR spectroscopy in combination with isotopomer analysis can be used to study the general dynamics of substrate A competition between carbohydrates and fats in rat skeletal muscle.

Original languageEnglish (US)
Pages (from-to)32-39
Number of pages8
JournalJournal of Applied Physiology
Volume83
Issue number1
StatePublished - Jul 1997

Fingerprint

Lactic Acid
Skeletal Muscle
Acetates
Magnetic Resonance Spectroscopy
Hindlimb
Carbohydrates
Radioactive Tracers
Muscle Contraction
Radioisotopes
Fatty Acids
Fats

Keywords

  • Acetate
  • Carbon-13-isotopomer analysis
  • Citric acid cycle
  • Lactate
  • Muscle contraction

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Oxidation of lactate and acetate in rat skeletal muscle : Analysis by 13C-nuclear magnetic resonance spectroscopy. / Bertocci, Loren A.; Jones, John G.; Malloy, Craig R.; Victor, Ronald G.; Thomas, Gail D.

In: Journal of Applied Physiology, Vol. 83, No. 1, 07.1997, p. 32-39.

Research output: Contribution to journalArticle

Bertocci, Loren A. ; Jones, John G. ; Malloy, Craig R. ; Victor, Ronald G. ; Thomas, Gail D. / Oxidation of lactate and acetate in rat skeletal muscle : Analysis by 13C-nuclear magnetic resonance spectroscopy. In: Journal of Applied Physiology. 1997 ; Vol. 83, No. 1. pp. 32-39.
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