P-selectin blockade is beneficial after uncontrolled hemorrhagic shock

Fernando Rivera-Chavez, Luis H. Toledo-Pereyra, Dean T. Nora, Ben Bachulis, Frederick Ilgenfritz, Richard E. Dean

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22 Scopus citations

Abstract

Objectives: The selectins play an important role in the neutrophil- mediated injury after hemorrhagic shock and resuscitation. The aim of this study was to investigate the effect of P-selectin blockade after HS and resuscitation. Methods: Forty-eight Sprague-Dawley rats were subjected to controlled combined with uncontrolled HS and resuscitation. Rats were divided into three groups (n = 16/group): (1) sham, no HS; (2) control, HS + resuscitation + drug vehicle; (3) treated, HS + anti-P-selectin monoclonal antibody. Transaminase levels to measure hepatocellular injury, liver myeloperoxidase to assess neutrophil infiltration, and histology were analyzed and compared between groups. Survival was followed for 3 days and was compared statistically. Results: Survival significantly increased from 30% in the control group to 70% in the treated group. Hepatocellular and structural injury as well as neutrophil infiltration were significantly decreased in treated animals. Conclusion: Blockade of P-selectin resulted in decreased hepatocellular injury and increased survival in our model of uncontrolled HS. Selectins may be important therapeutic targets for blockade in the treatment of HS.

Original languageEnglish (US)
Pages (from-to)440-445
Number of pages6
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume45
Issue number3
DOIs
StatePublished - Sep 1 1998

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Keywords

  • Ischemia/reperfusion
  • Liver
  • P-selectin
  • Uncontrolled hemorrhagic shock

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine

Cite this

Rivera-Chavez, F., Toledo-Pereyra, L. H., Nora, D. T., Bachulis, B., Ilgenfritz, F., & Dean, R. E. (1998). P-selectin blockade is beneficial after uncontrolled hemorrhagic shock. Journal of Trauma - Injury, Infection and Critical Care, 45(3), 440-445. https://doi.org/10.1097/00005373-199809000-00003