p31comet Blocks Mad2 Activation through Structural Mimicry

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131 Scopus citations

Abstract

The status of spindle checkpoint signaling depends on the balance of two opposing dynamic processes that regulate the highly unusual two-state behavior of Mad2. In mitosis, a Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores through Mad2 dimerization and converts Mad2 to a conformer amenable to Cdc20 binding, thereby facilitating checkpoint activation. p31comet inactivates the checkpoint through binding to Mad1- or Cdc20-bound Mad2, thereby preventing Mad2 activation and promoting the dissociation of the Mad2-Cdc20 complex. Here, we report the crystal structure of the Mad2-p31comet complex. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31comet binding, explaining the specificity of p31comet toward Mad1- or Cdc20-bound Mad2. p31comet adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31comet exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2.".

Original languageEnglish (US)
Pages (from-to)744-755
Number of pages12
JournalCell
Volume131
Issue number4
DOIs
StatePublished - Nov 16 2007

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Keywords

  • CELLCYCLE

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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