Palindromic sequences in heteroduplex DNA inhibit mismatch repair in yeast

Dilip K. Nag, Michael A. White, Thomas D. Petes

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

ALTHOUGH single heterozygous markers in yeast usually segregate during meiosis in a 2:2 ratio, abberant 3:1 segregations occur quite frequently as a result of gene-conversion events1. A second type of aberrant segregation, post-meiotic segregation, results from the segregation of two genotypes from a single haploid spore; in yeast such events are detected as sectored spore colonies and usually occur rarely1. Post-meiotic segregation is thought to result from the replication of heteroduplex DNA formed during meiotic recombination. We report here that if the heteroduplex includes a palindromic insertion sequence, a high frequency of post-meiotic segregation results. This suggests that palindromic insertions are poorly repaired, which may be the result of hairpin-loop formation that affects the efficiency of repair of heteroduplex DNA.

Original languageEnglish (US)
Pages (from-to)318-320
Number of pages3
JournalNature
Volume340
Issue number6231
StatePublished - 1989

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Nucleic Acid Heteroduplexes
DNA Mismatch Repair
Spores
Yeasts
Gene Conversion
Insertional Mutagenesis
Haploidy
Meiosis
Genetic Recombination
Genotype

ASJC Scopus subject areas

  • General

Cite this

Palindromic sequences in heteroduplex DNA inhibit mismatch repair in yeast. / Nag, Dilip K.; White, Michael A.; Petes, Thomas D.

In: Nature, Vol. 340, No. 6231, 1989, p. 318-320.

Research output: Contribution to journalArticle

Nag, DK, White, MA & Petes, TD 1989, 'Palindromic sequences in heteroduplex DNA inhibit mismatch repair in yeast', Nature, vol. 340, no. 6231, pp. 318-320.
Nag, Dilip K. ; White, Michael A. ; Petes, Thomas D. / Palindromic sequences in heteroduplex DNA inhibit mismatch repair in yeast. In: Nature. 1989 ; Vol. 340, No. 6231. pp. 318-320.
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