Pancreatic cancer cells overexpress gelsolin family-capping proteins, which contribute to their cell motility

C. C. Thompson, F. J. Ashcroft, S. Patel, G. Saraga, D. Vimalachandran, W. Prime, F. Campbell, A. Dodson, R. E. Jenkins, N. R. Lemoine, T. Crnogorac-Jurcevic, H. L. Yin, E. Costello

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Abstract

Background: Previously, proteomic methods were applied to characterise differentially expressed proteins in microdissected pancreatic ductal adenocarcinoma cells. Aims: To report that CapG and a related protein, gelsolin, which have established roles in cell motility, are overexpressed in metastatic pancreatic cancer; and to describe their pattern of expression in pancreatic cancer tissue and their effect on cell motility in pancreatic cancer cell lines. Methods: CapG was identified by mass spectrometry and immunoblotting. CapG and gelsolin expression was assessed by immunohistochemical analysis on a pancreatic cancer tissue microarray and correlated with clinical and pathological parameters. CapG and gelsolin levels were reduced using RNA interface in Suit-2, Panc-1 and MiaPaCa-2 cells. Cell motility was assessed using modified Boyden chamber or wound-healing assays. Results: Multiple isoforms of CapG were detected in pancreatic cancer tissue and cell lines. Immunohistochemical analysis of benign (n = 44 patients) and malignant (n = 69) pancreatic ductal cells showed significantly higher CapG staining intensity in nuclear (p<0.001) and cytoplasmic (p<0.001) compartments of malignant cells. Similarly, gelsolin immunostaining of benign (n = 24 patients) and malignant (n = 68 patients) pancreatic ductal cells showed higher expression in both compartments (both p<0.001 ). High nuclear CapG was associated with increased tumour size (p = 0.001 ). High nuclear gelsolin was associated with reduced survival (p = 0.01). Reduction of CapG or gelsolin expression in cell lines by RNAi was accompanied by significantly impaired motility. Conclusions: Up regulation of these actin-capping proteins in pancreatic cancer and their ability to modulate cell motility in vitro suggest their potentially important role in pancreatic cancer cell motility and consequently dissemination.

Original languageEnglish (US)
Pages (from-to)95-106
Number of pages12
JournalGut
Volume56
Issue number1
DOIs
StatePublished - Jan 2007

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Gelsolin
Pancreatic Neoplasms
Cell Movement
Proteins
Cell Line
Actin Capping Proteins
RNA Interference
Immunoblotting
Proteomics
Wound Healing
Mass Spectrometry
Protein Isoforms
Adenocarcinoma
Up-Regulation
RNA
Staining and Labeling
Survival

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Thompson, C. C., Ashcroft, F. J., Patel, S., Saraga, G., Vimalachandran, D., Prime, W., ... Costello, E. (2007). Pancreatic cancer cells overexpress gelsolin family-capping proteins, which contribute to their cell motility. Gut, 56(1), 95-106. https://doi.org/10.1136/gut.2005.083691

Pancreatic cancer cells overexpress gelsolin family-capping proteins, which contribute to their cell motility. / Thompson, C. C.; Ashcroft, F. J.; Patel, S.; Saraga, G.; Vimalachandran, D.; Prime, W.; Campbell, F.; Dodson, A.; Jenkins, R. E.; Lemoine, N. R.; Crnogorac-Jurcevic, T.; Yin, H. L.; Costello, E.

In: Gut, Vol. 56, No. 1, 01.2007, p. 95-106.

Research output: Contribution to journalArticle

Thompson, CC, Ashcroft, FJ, Patel, S, Saraga, G, Vimalachandran, D, Prime, W, Campbell, F, Dodson, A, Jenkins, RE, Lemoine, NR, Crnogorac-Jurcevic, T, Yin, HL & Costello, E 2007, 'Pancreatic cancer cells overexpress gelsolin family-capping proteins, which contribute to their cell motility', Gut, vol. 56, no. 1, pp. 95-106. https://doi.org/10.1136/gut.2005.083691
Thompson CC, Ashcroft FJ, Patel S, Saraga G, Vimalachandran D, Prime W et al. Pancreatic cancer cells overexpress gelsolin family-capping proteins, which contribute to their cell motility. Gut. 2007 Jan;56(1):95-106. https://doi.org/10.1136/gut.2005.083691
Thompson, C. C. ; Ashcroft, F. J. ; Patel, S. ; Saraga, G. ; Vimalachandran, D. ; Prime, W. ; Campbell, F. ; Dodson, A. ; Jenkins, R. E. ; Lemoine, N. R. ; Crnogorac-Jurcevic, T. ; Yin, H. L. ; Costello, E. / Pancreatic cancer cells overexpress gelsolin family-capping proteins, which contribute to their cell motility. In: Gut. 2007 ; Vol. 56, No. 1. pp. 95-106.
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abstract = "Background: Previously, proteomic methods were applied to characterise differentially expressed proteins in microdissected pancreatic ductal adenocarcinoma cells. Aims: To report that CapG and a related protein, gelsolin, which have established roles in cell motility, are overexpressed in metastatic pancreatic cancer; and to describe their pattern of expression in pancreatic cancer tissue and their effect on cell motility in pancreatic cancer cell lines. Methods: CapG was identified by mass spectrometry and immunoblotting. CapG and gelsolin expression was assessed by immunohistochemical analysis on a pancreatic cancer tissue microarray and correlated with clinical and pathological parameters. CapG and gelsolin levels were reduced using RNA interface in Suit-2, Panc-1 and MiaPaCa-2 cells. Cell motility was assessed using modified Boyden chamber or wound-healing assays. Results: Multiple isoforms of CapG were detected in pancreatic cancer tissue and cell lines. Immunohistochemical analysis of benign (n = 44 patients) and malignant (n = 69) pancreatic ductal cells showed significantly higher CapG staining intensity in nuclear (p<0.001) and cytoplasmic (p<0.001) compartments of malignant cells. Similarly, gelsolin immunostaining of benign (n = 24 patients) and malignant (n = 68 patients) pancreatic ductal cells showed higher expression in both compartments (both p<0.001 ). High nuclear CapG was associated with increased tumour size (p = 0.001 ). High nuclear gelsolin was associated with reduced survival (p = 0.01). Reduction of CapG or gelsolin expression in cell lines by RNAi was accompanied by significantly impaired motility. Conclusions: Up regulation of these actin-capping proteins in pancreatic cancer and their ability to modulate cell motility in vitro suggest their potentially important role in pancreatic cancer cell motility and consequently dissemination.",
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AU - Thompson, C. C.

AU - Ashcroft, F. J.

AU - Patel, S.

AU - Saraga, G.

AU - Vimalachandran, D.

AU - Prime, W.

AU - Campbell, F.

AU - Dodson, A.

AU - Jenkins, R. E.

AU - Lemoine, N. R.

AU - Crnogorac-Jurcevic, T.

AU - Yin, H. L.

AU - Costello, E.

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N2 - Background: Previously, proteomic methods were applied to characterise differentially expressed proteins in microdissected pancreatic ductal adenocarcinoma cells. Aims: To report that CapG and a related protein, gelsolin, which have established roles in cell motility, are overexpressed in metastatic pancreatic cancer; and to describe their pattern of expression in pancreatic cancer tissue and their effect on cell motility in pancreatic cancer cell lines. Methods: CapG was identified by mass spectrometry and immunoblotting. CapG and gelsolin expression was assessed by immunohistochemical analysis on a pancreatic cancer tissue microarray and correlated with clinical and pathological parameters. CapG and gelsolin levels were reduced using RNA interface in Suit-2, Panc-1 and MiaPaCa-2 cells. Cell motility was assessed using modified Boyden chamber or wound-healing assays. Results: Multiple isoforms of CapG were detected in pancreatic cancer tissue and cell lines. Immunohistochemical analysis of benign (n = 44 patients) and malignant (n = 69) pancreatic ductal cells showed significantly higher CapG staining intensity in nuclear (p<0.001) and cytoplasmic (p<0.001) compartments of malignant cells. Similarly, gelsolin immunostaining of benign (n = 24 patients) and malignant (n = 68 patients) pancreatic ductal cells showed higher expression in both compartments (both p<0.001 ). High nuclear CapG was associated with increased tumour size (p = 0.001 ). High nuclear gelsolin was associated with reduced survival (p = 0.01). Reduction of CapG or gelsolin expression in cell lines by RNAi was accompanied by significantly impaired motility. Conclusions: Up regulation of these actin-capping proteins in pancreatic cancer and their ability to modulate cell motility in vitro suggest their potentially important role in pancreatic cancer cell motility and consequently dissemination.

AB - Background: Previously, proteomic methods were applied to characterise differentially expressed proteins in microdissected pancreatic ductal adenocarcinoma cells. Aims: To report that CapG and a related protein, gelsolin, which have established roles in cell motility, are overexpressed in metastatic pancreatic cancer; and to describe their pattern of expression in pancreatic cancer tissue and their effect on cell motility in pancreatic cancer cell lines. Methods: CapG was identified by mass spectrometry and immunoblotting. CapG and gelsolin expression was assessed by immunohistochemical analysis on a pancreatic cancer tissue microarray and correlated with clinical and pathological parameters. CapG and gelsolin levels were reduced using RNA interface in Suit-2, Panc-1 and MiaPaCa-2 cells. Cell motility was assessed using modified Boyden chamber or wound-healing assays. Results: Multiple isoforms of CapG were detected in pancreatic cancer tissue and cell lines. Immunohistochemical analysis of benign (n = 44 patients) and malignant (n = 69) pancreatic ductal cells showed significantly higher CapG staining intensity in nuclear (p<0.001) and cytoplasmic (p<0.001) compartments of malignant cells. Similarly, gelsolin immunostaining of benign (n = 24 patients) and malignant (n = 68 patients) pancreatic ductal cells showed higher expression in both compartments (both p<0.001 ). High nuclear CapG was associated with increased tumour size (p = 0.001 ). High nuclear gelsolin was associated with reduced survival (p = 0.01). Reduction of CapG or gelsolin expression in cell lines by RNAi was accompanied by significantly impaired motility. Conclusions: Up regulation of these actin-capping proteins in pancreatic cancer and their ability to modulate cell motility in vitro suggest their potentially important role in pancreatic cancer cell motility and consequently dissemination.

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