PDGF-stimulated dispersal of cell clusters and disruption of fibronectin matrix on three-dimensional collagen matrices requires matrix metalloproteinase-2

Bruno Da Rocha-Azevedo, Chin Han Ho, Frederick Grinnell

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Formation of cell clusters is a common morphogenic cell behavior observed during tissue and organ development and homeostasis, as well as during pathological disorders. Dynamic regulation of cell clustering depends on the balance between contraction of cells into clusters and migration of cells as dispersed individuals. Previously we reported that under procontractile culture conditions, fibronectin fibrillar matrix assembly by human fibroblasts functioned as a nucleation center for cell clustering on three-dimensional collagen matrices. Here we report that switching preformed cell clusters from procontractile to promigratory culture conditions results in cell dispersal out of clusters and disruption of FN matrix. Experiments using small interfering RNA silencing and pharmacological inhibition demonstrated that matrix metalloproteinase activity involving MMP-2 was necessary for fibronectin matrix disruption and dispersal of cell clusters.

Original languageEnglish (US)
Pages (from-to)1098-1105
Number of pages8
JournalMolecular biology of the cell
Volume26
Issue number6
DOIs
StatePublished - Mar 15 2015

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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