Peroxisomal protein import is conserved between yeast, plants, insects and mammals

Stephen J. Gould, Gilbert Andre Keller, Michel Schneider, Stephen H. Howell, Lisa J. Garrard, Joel M. Goodman, Ben Distel, Henk Tabak, Suresh Subramani

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

We have previously demonstrated that firefly luciferase can be imported into peroxisomes of both insect and mammalian cells. To determine whether the process of protein transport into the peroxisome is functionally similar in more widely divergent eukaryotes, the cDNA encoding firefly luciferase was expressed in both yeast and plant cells. Luciferase was translocated into peroxisomes in each type of organism. Experiments were also performed to determine whether a yeast peroxisornal protein could be transported to peroxisomes in mammalian cells. We observed that a C-terminal segment of the yeast (Candida boidinii) peroxisomal protein PMP20 could act as a peroxisomal targeting signal in mammalian cells. These results suggest that at least one mechanism of protein translocation into peroxisomes has been conserved throughout eukaryotic evolution.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalEMBO Journal
Volume9
Issue number1
StatePublished - 1990

Fingerprint

Mammals
Peroxisomes
Yeast
Firefly Luciferases
Insects
Yeasts
Cells
Protein Transport
Proteins
Fungal Proteins
Candida
Luciferases
Complementary DNA
Plant Cells
Eukaryota
Experiments

Keywords

  • Firefly luciferase
  • Peroxisome
  • Translocation
  • Yeast

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Gould, S. J., Keller, G. A., Schneider, M., Howell, S. H., Garrard, L. J., Goodman, J. M., ... Subramani, S. (1990). Peroxisomal protein import is conserved between yeast, plants, insects and mammals. EMBO Journal, 9(1), 85-90.

Peroxisomal protein import is conserved between yeast, plants, insects and mammals. / Gould, Stephen J.; Keller, Gilbert Andre; Schneider, Michel; Howell, Stephen H.; Garrard, Lisa J.; Goodman, Joel M.; Distel, Ben; Tabak, Henk; Subramani, Suresh.

In: EMBO Journal, Vol. 9, No. 1, 1990, p. 85-90.

Research output: Contribution to journalArticle

Gould, SJ, Keller, GA, Schneider, M, Howell, SH, Garrard, LJ, Goodman, JM, Distel, B, Tabak, H & Subramani, S 1990, 'Peroxisomal protein import is conserved between yeast, plants, insects and mammals', EMBO Journal, vol. 9, no. 1, pp. 85-90.
Gould SJ, Keller GA, Schneider M, Howell SH, Garrard LJ, Goodman JM et al. Peroxisomal protein import is conserved between yeast, plants, insects and mammals. EMBO Journal. 1990;9(1):85-90.
Gould, Stephen J. ; Keller, Gilbert Andre ; Schneider, Michel ; Howell, Stephen H. ; Garrard, Lisa J. ; Goodman, Joel M. ; Distel, Ben ; Tabak, Henk ; Subramani, Suresh. / Peroxisomal protein import is conserved between yeast, plants, insects and mammals. In: EMBO Journal. 1990 ; Vol. 9, No. 1. pp. 85-90.
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