Peroxisomal protein import is conserved between yeast, plants, insects and mammals

Stephen J. Gould, Gilbert Andre Keller, Michel Schneider, Stephen H. Howell, Lisa J. Garrard, Joel M. Goodman, Ben Distel, Henk Tabak, Suresh Subramani

Research output: Contribution to journalArticle

215 Scopus citations

Abstract

We have previously demonstrated that firefly luciferase can be imported into peroxisomes of both insect and mammalian cells. To determine whether the process of protein transport into the peroxisome is functionally similar in more widely divergent eukaryotes, the cDNA encoding firefly luciferase was expressed in both yeast and plant cells. Luciferase was translocated into peroxisomes in each type of organism. Experiments were also performed to determine whether a yeast peroxisomal protein could be transported to peroxisomes in mammalian cells. We observed that a C-terminal segment of the yeast (candida boidinii) peroxisomal protein PMP20 could act as a peroxisomal targeting signal in mammalian cells. These results suggest that at least one mechanism of protein translocation into peroxisomes has been conserved throughout eukaryotic evolution.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalEMBO Journal
Volume9
Issue number1
DOIs
StatePublished - 1990

Keywords

  • firefly luciferase
  • peroxisome
  • translocation
  • yeast

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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    Gould, S. J., Keller, G. A., Schneider, M., Howell, S. H., Garrard, L. J., Goodman, J. M., Distel, B., Tabak, H., & Subramani, S. (1990). Peroxisomal protein import is conserved between yeast, plants, insects and mammals. EMBO Journal, 9(1), 85-90. https://doi.org/10.1002/j.1460-2075.1990.tb08083.x