pH tolerance of rabbit corneal epithelium in tissue culture

D. R. Meyer, James P McCulley

Research output: Contribution to journalArticle

Abstract

The pH tolerance of stratified rabbit corneal epithelial cultures was determined for a balanced salt solution and various pharmaceutical vehicles to determine the biocompatibility of the different formulations after 1-128 min exposures. Acute damage was assessed by monitoring the release of radiolabeled nucleotides and corresponding changes in tissue morphology of the prelabeled cultures. Protracted damage was evaluated by re-examining the morphology of the same cultures at 24 hr along with quantitative measurement of residual 3H release, tissue loss, and concomitant changes in protein synthesis. Our testing results indicated that the permissive limits of the balanced salt solution were between a pH of 6.0 and 8.0, which is comparable to the guidelines for native endothelium established by organ perfusion studies. In contrast, the permissive limits of prototypes of ''intraocular'' vehicles were frequently more restricted, since only buffered solutions at neutral pH were relatively nontoxic. Most conventional ''topical'' vehicles, by comparison, were much more damaging, which indicates that pH is a more critical factor in these types of formulations. These data indicate that this type of bioassay can be effectively used for screening in drug development programs, and for establishing accurate guidelines for pharmaceutical formulations.

Original languageEnglish (US)
Pages (from-to)43-57
Number of pages15
JournalJournal of Toxicology - Cutaneous and Ocular Toxicology
Volume10
Issue number1-2
StatePublished - 1991

Fingerprint

Tissue culture
Corneal Epithelium
Rabbits
Pharmaceutical Vehicles
Salts
Tissue
Bioassay
Guidelines
Biocompatibility
Pharmaceutical Preparations
Drug Compounding
Preclinical Drug Evaluations
Screening
Nucleotides
Biological Assay
Endothelium
Perfusion
Monitoring
Testing
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

pH tolerance of rabbit corneal epithelium in tissue culture. / Meyer, D. R.; McCulley, James P.

In: Journal of Toxicology - Cutaneous and Ocular Toxicology, Vol. 10, No. 1-2, 1991, p. 43-57.

Research output: Contribution to journalArticle

@article{b8ee71eeff814e34b75b416988b443a4,
title = "pH tolerance of rabbit corneal epithelium in tissue culture",
abstract = "The pH tolerance of stratified rabbit corneal epithelial cultures was determined for a balanced salt solution and various pharmaceutical vehicles to determine the biocompatibility of the different formulations after 1-128 min exposures. Acute damage was assessed by monitoring the release of radiolabeled nucleotides and corresponding changes in tissue morphology of the prelabeled cultures. Protracted damage was evaluated by re-examining the morphology of the same cultures at 24 hr along with quantitative measurement of residual 3H release, tissue loss, and concomitant changes in protein synthesis. Our testing results indicated that the permissive limits of the balanced salt solution were between a pH of 6.0 and 8.0, which is comparable to the guidelines for native endothelium established by organ perfusion studies. In contrast, the permissive limits of prototypes of ''intraocular'' vehicles were frequently more restricted, since only buffered solutions at neutral pH were relatively nontoxic. Most conventional ''topical'' vehicles, by comparison, were much more damaging, which indicates that pH is a more critical factor in these types of formulations. These data indicate that this type of bioassay can be effectively used for screening in drug development programs, and for establishing accurate guidelines for pharmaceutical formulations.",
author = "Meyer, {D. R.} and McCulley, {James P}",
year = "1991",
language = "English (US)",
volume = "10",
pages = "43--57",
journal = "Cutaneous and Ocular Toxicology",
issn = "1556-9527",
publisher = "Informa Healthcare",
number = "1-2",

}

TY - JOUR

T1 - pH tolerance of rabbit corneal epithelium in tissue culture

AU - Meyer, D. R.

AU - McCulley, James P

PY - 1991

Y1 - 1991

N2 - The pH tolerance of stratified rabbit corneal epithelial cultures was determined for a balanced salt solution and various pharmaceutical vehicles to determine the biocompatibility of the different formulations after 1-128 min exposures. Acute damage was assessed by monitoring the release of radiolabeled nucleotides and corresponding changes in tissue morphology of the prelabeled cultures. Protracted damage was evaluated by re-examining the morphology of the same cultures at 24 hr along with quantitative measurement of residual 3H release, tissue loss, and concomitant changes in protein synthesis. Our testing results indicated that the permissive limits of the balanced salt solution were between a pH of 6.0 and 8.0, which is comparable to the guidelines for native endothelium established by organ perfusion studies. In contrast, the permissive limits of prototypes of ''intraocular'' vehicles were frequently more restricted, since only buffered solutions at neutral pH were relatively nontoxic. Most conventional ''topical'' vehicles, by comparison, were much more damaging, which indicates that pH is a more critical factor in these types of formulations. These data indicate that this type of bioassay can be effectively used for screening in drug development programs, and for establishing accurate guidelines for pharmaceutical formulations.

AB - The pH tolerance of stratified rabbit corneal epithelial cultures was determined for a balanced salt solution and various pharmaceutical vehicles to determine the biocompatibility of the different formulations after 1-128 min exposures. Acute damage was assessed by monitoring the release of radiolabeled nucleotides and corresponding changes in tissue morphology of the prelabeled cultures. Protracted damage was evaluated by re-examining the morphology of the same cultures at 24 hr along with quantitative measurement of residual 3H release, tissue loss, and concomitant changes in protein synthesis. Our testing results indicated that the permissive limits of the balanced salt solution were between a pH of 6.0 and 8.0, which is comparable to the guidelines for native endothelium established by organ perfusion studies. In contrast, the permissive limits of prototypes of ''intraocular'' vehicles were frequently more restricted, since only buffered solutions at neutral pH were relatively nontoxic. Most conventional ''topical'' vehicles, by comparison, were much more damaging, which indicates that pH is a more critical factor in these types of formulations. These data indicate that this type of bioassay can be effectively used for screening in drug development programs, and for establishing accurate guidelines for pharmaceutical formulations.

UR - http://www.scopus.com/inward/record.url?scp=0025986683&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025986683&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0025986683

VL - 10

SP - 43

EP - 57

JO - Cutaneous and Ocular Toxicology

JF - Cutaneous and Ocular Toxicology

SN - 1556-9527

IS - 1-2

ER -