Phenotypic and molecular characterization of proliferating and differentiated GnRH-expressing GnV-3 cells

Virginie Mansuy, Sarah Geller, Jean Pierre Rey, Céline Campagne, Julien Boccard, Pierre Poulain, Vincent Prevot, François P. Pralong

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

GnRH neurons provide the primary driving force upon the neuroendocrine reproductive axis. Here we used GnV-3 cells, a model of conditionally immortalized GnRH-expressing neurons, to perform an analysis of cell cycle and compare the gene expression profile of proliferating cells with differentiated cells.In the proliferation medium, 45 ± 1.5% of GnV-3 cells are in S-phase by FACS analysis. In the differentiation medium, only 9 ± 0.9% of them are in S-phase, and they acquire the characteristic bipolar shape displayed by preoptic GnRH neurons in vivo. In addition, GnV-3 cells in the differentiated state exhibit electrophysiological properties characteristic of neurons. Transcriptomic analysis identified up-regulation of 1931 genes and down-regulation of 1270 genes in cells grown in the differentiation medium compared to cells in the proliferation medium. Subsequent gene ontology study indicated that genes over-expressed in proliferating GnV-3 cells were mainly involved in cell cycle regulations, whereas genes over-expressed in differentiated cells were mainly involved in processes of differentiation, neurogenesis and neuronal morphogenesis. Taken together, these data demonstrate the occurrence of morphological and physiological changes in GnV-3 cells between the proliferating and the differentiated state. Moreover, the genes differentially regulated between these two different states are providing novel pathways potentially important for a better understanding of the physiology of mature GnRH neurons.

Original languageEnglish (US)
Pages (from-to)97-105
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume332
Issue number1-2
DOIs
StatePublished - Jan 30 2011

Keywords

  • Cell line
  • Differentiation
  • GnRH
  • Hypothalamus
  • Proliferation
  • Transcriptomic

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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