Phosphatidylserine exposure on the surface of Leishmania amazonensis amastigotes modulates in vivo infection and dendritic cell function

J. L M Wanderley, P. E. Thorpe, M. A. Barcinski, L. Soong

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Leishmania amazonensis parasites can cause diverse forms of leishmaniasis in humans and persistent lesions in most inbred strains of mice. In both cases, the infection is characterized by a marked immunosuppression of the host. We previously showed that amastigote forms of the parasite make use of surface-exposed phosphatidylserine (PS) molecules to infect host cells and promote alternative macrophage activation, leading to uncontrolled intracellular proliferation of the parasites. In this study, we demonstrated that treatment of infected mice with a PS-targeting monoclonal antibody ameliorated parasite loads and lesion development, which correlated with increased proliferative responses by lymphocytes. In addition, we observed an enhanced dendritic cell (DC) activation and antigen presentation in vitro. Our data imply that the recognition of PS exposed on the surface of amastigotes plays a role in down-modulating DC functions, in a matter similar to that of apoptotic cell clearance. This study provides new information regarding the mechanism of immune suppression in Leishmania infection.

Original languageEnglish (US)
Pages (from-to)109-119
Number of pages11
JournalParasite Immunology
Volume35
Issue number3-4
DOIs
StatePublished - Mar 1 2013

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Keywords

  • Dendritic cell
  • Immune evasion
  • Leishmania spp
  • Leishmaniasis

ASJC Scopus subject areas

  • Parasitology
  • Immunology

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