Phosphoinositide turnover signaling stimulated by ET-3 in endothelial cells from spontaneously hypertensive rats

K. Yokokawa, J. Johnson, M. Kohno, A. K. Mandal, M. Yanagisawa, T. Horio, K. Yasunari, T. Takeda

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Endothelin (ET) B-type receptor-mediated signal transduction after stimulation with ET-3 was examined in cultured aortic endothelial cells obtained from spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats. The purpose of this study was to elucidate ET(B) receptor- mediated response in endothelial cells from hypertensive rat models. Non- isopeptide-selective displacement and affinity in these binding experiments suggest that aortic endothelial cell receptors for ET-3 correspond to ET(B) receptor subtypes. These receptors for ET-3 were similar in WKY and SHR endothelial cells. ET(B) receptor mRNA expression in cultured endothelial cells was also similar in WKY and SHR. However, the cytosolic free-Ca2+ level in the absence of extracellular Ca2+ as well as the inositol 1,4,5- trisphosphate level in response to ET-3 were greater in endothelial cells from SHR than in those from WKY. Phospholipase C and protein kinase C activities after stimulation with ET-3 were also greater in SHR than in WKY. The 6-ketoprostaglandin F(1α) production was also augmented in SHR, although nitric oxide formation and guanosine 3',5'-cyclic monophosphate production after stimulation with ET-3 were similar in WKY and SHR. We conclude that the phosphoinositide turnover signaling stimulated by ET-3 is augmented in cultured aortic endothelial cells from SHR compared with those from WKY.

Original languageEnglish (US)
Pages (from-to)R635-R644
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume267
Issue number3 36-3
StatePublished - Jan 1 1994

Keywords

  • endothelin-3
  • phospholipase C
  • receptor

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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