Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells

Tatsuya Sawamura; Yoshitoshi Kasuya; Yohichi Matsushita; Nobuhiro Suzuki; Osamu Shinmi; Naoya Kishi; Yoshiki Sugita; Masashi Yanagisawa; Katsutoshi Goto; Tomoh Masaki; Sadao Kimura

doi:10.1016/0006-291X(91)91485-U

Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells

Tatsuya Sawamura, Yoshitoshi Kasuya, Yohichi Matsushita, Nobuhiro Suzuki, Osamu Shinmi, Naoya Kishi, Yoshiki Sugita, Masashi Yanagisawa, Katsutoshi Goto, Tomoh Masaki, Sadao Kimura

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Abstract

Effects of various protease inhibitors on the conversion of big endothelin (ET)-1 to ET-1 in cultured endothelial cells were analyzed. A metal protease inhibitor, phosphoramidon, decreases the amount of ET-1 and increases that of big ET-1 released. This effect is dose-dependent and not nonspecific. When the contents of ET-1 and big ET-1 in the cells after culturing in the medium with or without phosphoramidon were measured, the ratio of ET-1 : big ET-1 in the cells was 3.3 : 1 and phosphoramidon inverted the ratio in the cells to 1 : 3.5. These data strongly suggest that a phosphoramidon-sensitive protease converts big ET-1 to mature ET-1 intracellularly.

Original language	English (US)
Pages (from-to)	779-784
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	174
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(91)91485-U
State	Published - Jan 31 1991

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Sawamura, T., Kasuya, Y., Matsushita, Y., Suzuki, N., Shinmi, O., Kishi, N., Sugita, Y., Yanagisawa, M., Goto, K., Masaki, T., & Kimura, S. (1991). Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells. Biochemical and Biophysical Research Communications, 174(2), 779-784. https://doi.org/10.1016/0006-291X(91)91485-U

Sawamura, T, Kasuya, Y, Matsushita, Y, Suzuki, N, Shinmi, O, Kishi, N, Sugita, Y, Yanagisawa, M, Goto, K, Masaki, T & Kimura, S 1991, 'Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells', Biochemical and Biophysical Research Communications, vol. 174, no. 2, pp. 779-784. https://doi.org/10.1016/0006-291X(91)91485-U

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title = "Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells",

abstract = "Effects of various protease inhibitors on the conversion of big endothelin (ET)-1 to ET-1 in cultured endothelial cells were analyzed. A metal protease inhibitor, phosphoramidon, decreases the amount of ET-1 and increases that of big ET-1 released. This effect is dose-dependent and not nonspecific. When the contents of ET-1 and big ET-1 in the cells after culturing in the medium with or without phosphoramidon were measured, the ratio of ET-1 : big ET-1 in the cells was 3.3 : 1 and phosphoramidon inverted the ratio in the cells to 1 : 3.5. These data strongly suggest that a phosphoramidon-sensitive protease converts big ET-1 to mature ET-1 intracellularly.",

author = "Tatsuya Sawamura and Yoshitoshi Kasuya and Yohichi Matsushita and Nobuhiro Suzuki and Osamu Shinmi and Naoya Kishi and Yoshiki Sugita and Masashi Yanagisawa and Katsutoshi Goto and Tomoh Masaki and Sadao Kimura",

note = "Funding Information: This work was in part supported by grants from University of Tsukuba Project Research, the Ministry of Education, Science and Culture of Japan, and National Cardiovascular Center Research Institute.",

year = "1991",

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doi = "10.1016/0006-291X(91)91485-U",

language = "English (US)",

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T1 - Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells

AU - Sawamura, Tatsuya

AU - Kasuya, Yoshitoshi

AU - Matsushita, Yohichi

AU - Suzuki, Nobuhiro

AU - Shinmi, Osamu

AU - Kishi, Naoya

AU - Sugita, Yoshiki

AU - Yanagisawa, Masashi

AU - Goto, Katsutoshi

AU - Masaki, Tomoh

AU - Kimura, Sadao

N1 - Funding Information: This work was in part supported by grants from University of Tsukuba Project Research, the Ministry of Education, Science and Culture of Japan, and National Cardiovascular Center Research Institute.

PY - 1991/1/31

Y1 - 1991/1/31

N2 - Effects of various protease inhibitors on the conversion of big endothelin (ET)-1 to ET-1 in cultured endothelial cells were analyzed. A metal protease inhibitor, phosphoramidon, decreases the amount of ET-1 and increases that of big ET-1 released. This effect is dose-dependent and not nonspecific. When the contents of ET-1 and big ET-1 in the cells after culturing in the medium with or without phosphoramidon were measured, the ratio of ET-1 : big ET-1 in the cells was 3.3 : 1 and phosphoramidon inverted the ratio in the cells to 1 : 3.5. These data strongly suggest that a phosphoramidon-sensitive protease converts big ET-1 to mature ET-1 intracellularly.

AB - Effects of various protease inhibitors on the conversion of big endothelin (ET)-1 to ET-1 in cultured endothelial cells were analyzed. A metal protease inhibitor, phosphoramidon, decreases the amount of ET-1 and increases that of big ET-1 released. This effect is dose-dependent and not nonspecific. When the contents of ET-1 and big ET-1 in the cells after culturing in the medium with or without phosphoramidon were measured, the ratio of ET-1 : big ET-1 in the cells was 3.3 : 1 and phosphoramidon inverted the ratio in the cells to 1 : 3.5. These data strongly suggest that a phosphoramidon-sensitive protease converts big ET-1 to mature ET-1 intracellularly.

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Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells

Abstract

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