Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells

Tatsuya Sawamura, Yoshitoshi Kasuya, Yohichi Matsushita, Nobuhiro Suzuki, Osamu Shinmi, Naoya Kishi, Yoshiki Sugita, Masashi Yanagisawa, Katsutoshi Goto, Tomoh Masaki, Sadao Kimura

Research output: Contribution to journalArticle

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Abstract

Effects of various protease inhibitors on the conversion of big endothelin (ET)-1 to ET-1 in cultured endothelial cells were analyzed. A metal protease inhibitor, phosphoramidon, decreases the amount of ET-1 and increases that of big ET-1 released. This effect is dose-dependent and not nonspecific. When the contents of ET-1 and big ET-1 in the cells after culturing in the medium with or without phosphoramidon were measured, the ratio of ET-1 : big ET-1 in the cells was 3.3 : 1 and phosphoramidon inverted the ratio in the cells to 1 : 3.5. These data strongly suggest that a phosphoramidon-sensitive protease converts big ET-1 to mature ET-1 intracellularly.

Original languageEnglish (US)
Pages (from-to)779-784
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume174
Issue number2
DOIs
StatePublished - Jan 31 1991

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Endothelial cells
Endothelin-1
Cultured Cells
Endothelial Cells
Protease Inhibitors
phosphoramidon
Peptide Hydrolases
Metals

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells. / Sawamura, Tatsuya; Kasuya, Yoshitoshi; Matsushita, Yohichi; Suzuki, Nobuhiro; Shinmi, Osamu; Kishi, Naoya; Sugita, Yoshiki; Yanagisawa, Masashi; Goto, Katsutoshi; Masaki, Tomoh; Kimura, Sadao.

In: Biochemical and Biophysical Research Communications, Vol. 174, No. 2, 31.01.1991, p. 779-784.

Research output: Contribution to journalArticle

Sawamura, T, Kasuya, Y, Matsushita, Y, Suzuki, N, Shinmi, O, Kishi, N, Sugita, Y, Yanagisawa, M, Goto, K, Masaki, T & Kimura, S 1991, 'Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells', Biochemical and Biophysical Research Communications, vol. 174, no. 2, pp. 779-784. https://doi.org/10.1016/0006-291X(91)91485-U
Sawamura, Tatsuya ; Kasuya, Yoshitoshi ; Matsushita, Yohichi ; Suzuki, Nobuhiro ; Shinmi, Osamu ; Kishi, Naoya ; Sugita, Yoshiki ; Yanagisawa, Masashi ; Goto, Katsutoshi ; Masaki, Tomoh ; Kimura, Sadao. / Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 174, No. 2. pp. 779-784.
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