TY - JOUR
T1 - Photo-activatable probes for the analysis of receptor function in living cells.
AU - Li, Wen Hong
PY - 2010
Y1 - 2010
N2 - Photo-activatable (caged) probes are powerful research tools for biological investigation. The superb maneuverability of a light beam allows researchers to activate caged probes with pinpoint accuracy. Recent developments in caging chemistry and two-photon excitation technique further enhance our capability to perform photo-uncaging with even higher spatial and temporal resolution, offering new photonic approaches to study cell signaling dynamics in greater detail. Here we present a sample method that combines the techniques of photo-activation and digital fluorescence microscopy to assay an important class of intracellular receptors for the second messenger D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P(3), or IP(3)). The imaging assay is performed in fully intact living cells using a caged and cell membrane permeable ester derivative of IP(3), cm-IP(3)/PM.
AB - Photo-activatable (caged) probes are powerful research tools for biological investigation. The superb maneuverability of a light beam allows researchers to activate caged probes with pinpoint accuracy. Recent developments in caging chemistry and two-photon excitation technique further enhance our capability to perform photo-uncaging with even higher spatial and temporal resolution, offering new photonic approaches to study cell signaling dynamics in greater detail. Here we present a sample method that combines the techniques of photo-activation and digital fluorescence microscopy to assay an important class of intracellular receptors for the second messenger D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P(3), or IP(3)). The imaging assay is performed in fully intact living cells using a caged and cell membrane permeable ester derivative of IP(3), cm-IP(3)/PM.
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U2 - 10.1007/978-1-60761-404-3_6
DO - 10.1007/978-1-60761-404-3_6
M3 - Article
C2 - 19957126
AN - SCOPUS:77449123821
SN - 1064-3745
VL - 591
SP - 105
EP - 120
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -