Photo-activatable probes for the analysis of receptor function in living cells.

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Photo-activatable (caged) probes are powerful research tools for biological investigation. The superb maneuverability of a light beam allows researchers to activate caged probes with pinpoint accuracy. Recent developments in caging chemistry and two-photon excitation technique further enhance our capability to perform photo-uncaging with even higher spatial and temporal resolution, offering new photonic approaches to study cell signaling dynamics in greater detail. Here we present a sample method that combines the techniques of photo-activation and digital fluorescence microscopy to assay an important class of intracellular receptors for the second messenger D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P(3), or IP(3)). The imaging assay is performed in fully intact living cells using a caged and cell membrane permeable ester derivative of IP(3), cm-IP(3)/PM.

Original languageEnglish (US)
Pages (from-to)105-120
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume591
StatePublished - 2010

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Optics and Photonics
Inositol 1,4,5-Trisphosphate
Second Messenger Systems
Photons
Fluorescence Microscopy
Esters
Research Personnel
Cell Membrane
Light
Research

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Photo-activatable probes for the analysis of receptor function in living cells. / Li, Wen Hong.

In: Methods in molecular biology (Clifton, N.J.), Vol. 591, 2010, p. 105-120.

Research output: Contribution to journalArticle

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