Photocrosslinking approaches to interactome mapping

Nam D. Pham, Randy B. Parker, Jennifer J. Kohler

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Photocrosslinking approaches can be used to map interactome networks within the context of living cells. Photocrosslinking methods rely on use of metabolic engineering or genetic code expansion to incorporate photocrosslinking analogs of amino acids or sugars into cellular biomolecules. Immunological and mass spectrometry techniques are used to analyze crosslinked complexes, thereby defining specific interactomes. Because photocrosslinking can be conducted in native, cellular settings, it can be used to define context-dependent interactions. Photocrosslinking methods are also ideally suited for determining interactome dynamics, mapping interaction interfaces, and identifying transient interactions in which intrinsically disordered proteins and glycoproteins engage. Here we discuss the application of cell-based photocrosslinking to the study of specific problems in immune cell signaling, transcription, membrane protein dynamics, nucleocytoplasmic transport, and chaperone-assisted protein folding.

Original languageEnglish (US)
Pages (from-to)90-101
Number of pages12
JournalCurrent Opinion in Chemical Biology
Volume17
Issue number1
DOIs
Publication statusPublished - Feb 2013

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ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry

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