PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution

Yuwen Ke, Jae Wan Huh, Ross Warrington, Bing Li, Nan Wu, Mei Leng, Junmei Zhang, Haydn L. Ball, Hongtao Yu

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Centromeres nucleate the formation of kinetochores and are vital for chromosome segregation during mitosis. The SNF2 family helicase PICH (Plk1-interacting checkpoint helicase) and the BLM (the Bloom's syndrome protein) helicase decorate ultrafine histone-negative DNA threads that link the segregating sister centromeres during anaphase. The functions of PICH and BLM at these threads are not understood, however. Here, we show that PICH binds to BLM and enables BLM localization to anaphase centromeric threads. PICH-or BLM-RNAi cells fail to resolve these threads in anaphase. The fragmented threads form centromeric-chromatin-containing micronuclei in daughter cells. Anaphase threads in PICH-and BLM-RNAi cells contain histones and centromere markers. Recombinant purified PICH has nucleosome remodelling activities in vitro. We propose that PICH and BLM unravel centromeric chromatin and keep anaphase DNA threads mostly free of nucleosomes, thus allowing these threads to span long distances between rapidly segregating centromeres without breakage and providing a spatiotemporal window for their resolution.

Original languageEnglish (US)
Pages (from-to)3309-3321
Number of pages13
JournalEMBO Journal
Volume30
Issue number16
DOIs
StatePublished - Aug 17 2011

Fingerprint

Anaphase
Nucleosomes
Histones
Centromere
Chromatin
DNA
Chromosomes
RNA Interference
Kinetochores
Chromosome Segregation
Mitosis
Ultrafine
Bloom syndrome protein

Keywords

  • cell centromere
  • chromatin remodelling
  • DNA repair
  • mitosis

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Medicine(all)

Cite this

PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution. / Ke, Yuwen; Huh, Jae Wan; Warrington, Ross; Li, Bing; Wu, Nan; Leng, Mei; Zhang, Junmei; Ball, Haydn L.; Yu, Hongtao.

In: EMBO Journal, Vol. 30, No. 16, 17.08.2011, p. 3309-3321.

Research output: Contribution to journalArticle

Ke, Yuwen ; Huh, Jae Wan ; Warrington, Ross ; Li, Bing ; Wu, Nan ; Leng, Mei ; Zhang, Junmei ; Ball, Haydn L. ; Yu, Hongtao. / PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution. In: EMBO Journal. 2011 ; Vol. 30, No. 16. pp. 3309-3321.
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