Polypeptide release factor eRF1 from Tetrahymena thermophila: cDNA cloning, purification and complex formation with yeast eRF3

Andrew L. Karamyshev, Koichi Ito, Yoshikazu Nakamura

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The first cDNA for the translational release factor eRF1 of ciliates was cloned from Tetrahymena thermophila. The coding frame contained one UAG and nine UAA codons that are reassigned for glutamine in Tetrahymena. The deduced protein sequence is 57% identical to human eRF1. The recombinant Tetrahymena eRF1 purified from a yeast expression system was able to bind to yeast eRF3 as do other yeast or mammalian eRF1s as a prerequisite step for protein termination. The recombinant Tetrahymena eRF1, nevertheless, failed to catalyze polypeptide termination in vitro with rat or Artemia ribosomes, at least in part, due to less efficient binding to the heterologous ribosomes. Stop codon specificity and phylogenetic significance of Tetrahymena eRF1 are discussed from the conservative protein feature. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)483-488
Number of pages6
JournalFEBS Letters
Volume457
Issue number3
DOIs
StatePublished - Sep 3 1999

Fingerprint

Tetrahymena thermophila
Tetrahymena
Cloning
Yeast
Purification
Organism Cloning
Complementary DNA
Yeasts
Peptides
Terminator Codon
Ribosomes
Proteins
Glutamine
Artemia
Rats

Keywords

  • eRF1-eRF3 complex
  • Polypeptide release factor
  • Tetrahymena thermophila
  • Translation termination
  • UGA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Polypeptide release factor eRF1 from Tetrahymena thermophila : cDNA cloning, purification and complex formation with yeast eRF3. / Karamyshev, Andrew L.; Ito, Koichi; Nakamura, Yoshikazu.

In: FEBS Letters, Vol. 457, No. 3, 03.09.1999, p. 483-488.

Research output: Contribution to journalArticle

Karamyshev, Andrew L. ; Ito, Koichi ; Nakamura, Yoshikazu. / Polypeptide release factor eRF1 from Tetrahymena thermophila : cDNA cloning, purification and complex formation with yeast eRF3. In: FEBS Letters. 1999 ; Vol. 457, No. 3. pp. 483-488.
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