Porcine factor V: cDNA cloning, gene mapping, three-dimensional protein modeling of membrane binding sites and comparative anatomy of domains

D. R. Grimm, M. B. Colter, M. Braunschweig, L. J. Alexander, P. J. Neame, H. K W Kim

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Factor V is a plasma protein essential for blood coagulation. This protein is involved in activated protein C resistance, the most common inherited thrombotic disorder known. We utilized the polymerase chain reaction to clone the porcine factor V gene by generating overlapping clones amplified with primers chosen by comparison with known nucleotide sequences. The porcine factor V cDNA contig encodes a predicted 2258-amino acid protein, making it the largest in comparison to the bovine, human, and murine proteins. Porcine factor V has the highest level of homology with bovine factor V, but also has high levels of conservation of important residues with all the species. Radiation hybrid mapping assigned the porcine factor V gene to chromosome 4. Three-dimensional models of factor V were generated and used to analyze membrane-binding sites in terms of conserved, and therefore likely important residues.

Original languageEnglish (US)
Pages (from-to)148-159
Number of pages12
JournalCellular and Molecular Life Sciences
Volume58
Issue number1
DOIs
StatePublished - Jan 1 2001

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Keywords

  • Cloning
  • Comparative anatomy
  • Factor V
  • Gene mapping
  • Modeling
  • Porcine

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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