Porcine factor V

cDNA cloning, gene mapping, three-dimensional protein modeling of membrane binding sites and comparative anatomy of domains

D. R. Grimm, M. B. Colter, M. Braunschweig, L. J. Alexander, P. J. Neame, H. K W Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Factor V is a plasma protein essential for blood coagulation. This protein is involved in activated protein C resistance, the most common inherited thrombotic disorder known. We utilized the polymerase chain reaction to clone the porcine factor V gene by generating overlapping clones amplified with primers chosen by comparison with known nucleotide sequences. The porcine factor V cDNA contig encodes a predicted 2258-amino acid protein, making it the largest in comparison to the bovine, human, and murine proteins. Porcine factor V has the highest level of homology with bovine factor V, but also has high levels of conservation of important residues with all the species. Radiation hybrid mapping assigned the porcine factor V gene to chromosome 4. Three-dimensional models of factor V were generated and used to analyze membrane-binding sites in terms of conserved, and therefore likely important residues.

Original languageEnglish (US)
Pages (from-to)148-159
Number of pages12
JournalCellular and Molecular Life Sciences
Volume58
Issue number1
StatePublished - 2001

Fingerprint

Comparative Anatomy
Factor V
Chromosome Mapping
Cloning
Organism Cloning
Membrane Proteins
Swine
Complementary DNA
Genes
Binding Sites
Membranes
Proteins
Clone Cells
Overlapping Genes
Radiation Hybrid Mapping
Activated Protein C Resistance
Chromosomes, Human, Pair 4
Polymerase chain reaction
Blood Coagulation
Chromosomes

Keywords

  • Cloning
  • Comparative anatomy
  • Factor V
  • Gene mapping
  • Modeling
  • Porcine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Porcine factor V : cDNA cloning, gene mapping, three-dimensional protein modeling of membrane binding sites and comparative anatomy of domains. / Grimm, D. R.; Colter, M. B.; Braunschweig, M.; Alexander, L. J.; Neame, P. J.; Kim, H. K W.

In: Cellular and Molecular Life Sciences, Vol. 58, No. 1, 2001, p. 148-159.

Research output: Contribution to journalArticle

Grimm, D. R. ; Colter, M. B. ; Braunschweig, M. ; Alexander, L. J. ; Neame, P. J. ; Kim, H. K W. / Porcine factor V : cDNA cloning, gene mapping, three-dimensional protein modeling of membrane binding sites and comparative anatomy of domains. In: Cellular and Molecular Life Sciences. 2001 ; Vol. 58, No. 1. pp. 148-159.
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