Potential role of bFGF and retinoic acid in the regeneration of chicken cochlear hair cells

Kenneth H. Lee, Douglas A. Cotanche

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Messenger RNAs (mRNA) of several growth factor receptors and related genes were examined with reverse transcriptase polymerase chain reaction (RT-PCR) in normal and noise-damaged chicken basilar papillae (BP). Analysis of the amplification products indicated the presence of mRNAs for epidermal growth factor receptor (EGFR), fibroblast growth factor receptor (FGFR), insulin-like growth factor receptor (IGFR), insulin receptor (IR), retinoic acid receptor beta (RARβ), retinoic acid receptor gamma (RXRγ), and basic fibroblast growth factor (BFGF) in both normal and noise-damaged BP. The RT-PCR products generated were characterized by size and sequencing analysis to confirm the identities of the target molecules. The subcellular localization of the mature protein analogs for EGFR, FGFR, IGFR, RARβ, and BFGF were identified using fluorescence immunocytochemistry and confocal laser scanning microscopy. These experiments indicated that EGFR is present in the stereociliary bundles in the hair cells, IGFR is not present in the cells of the BP, BFGF localizes in the nuclei of supporting cells in the BP, but not hair cells or hyaline cells, and that RARβ localizes in the perinuclear regions of hair cells. The subcellular distributions of these proteins were consistent in both noise-damaged and control BP. FGFR, in contrast, changed its distribution in the tissue after noise damage. In normal BP, FGFR is concentrated in the stereocilia of hair cells. However, in damaged regions of noise-exposed chick cochleae, FGFR is heavily expressed in the expanded apical regions of the supporting cells. These findings suggest that BFGF and retinoic acid may potentially play a role in the mechanisms which regulate the regeneration of chicken cochlear hair cells.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalHearing Research
Volume94
Issue number1-2
DOIs
StatePublished - May 1996

Fingerprint

Auditory Hair Cells
Organ of Corti
Tretinoin
Fibroblast Growth Factor Receptors
Regeneration
Chickens
Noise
Fibroblast Growth Factor 2
Somatomedin Receptors
Epidermal Growth Factor Receptor
Reverse Transcriptase Polymerase Chain Reaction
Stereocilia
Messenger RNA
Hyalin
Growth Factor Receptors
Cochlea
Insulin Receptor
Tissue Distribution
Cell Nucleus
Confocal Microscopy

Keywords

  • Basic fibroblast growth factor
  • Chicken
  • Cochlea
  • Epidermal growth factor receptor
  • Fibroblast growth factor response
  • Hair cell
  • Insulin receptor
  • Insulin-like growth factor receptor
  • Regeneration
  • Retinoic acid
  • Retinoic acid receptor beta
  • Retinoic acid receptor gamma
  • Reverse transcriptase PCR

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Potential role of bFGF and retinoic acid in the regeneration of chicken cochlear hair cells. / Lee, Kenneth H.; Cotanche, Douglas A.

In: Hearing Research, Vol. 94, No. 1-2, 05.1996, p. 1-13.

Research output: Contribution to journalArticle

@article{48856631d6e0462fb548a49e6842a966,
title = "Potential role of bFGF and retinoic acid in the regeneration of chicken cochlear hair cells",
abstract = "Messenger RNAs (mRNA) of several growth factor receptors and related genes were examined with reverse transcriptase polymerase chain reaction (RT-PCR) in normal and noise-damaged chicken basilar papillae (BP). Analysis of the amplification products indicated the presence of mRNAs for epidermal growth factor receptor (EGFR), fibroblast growth factor receptor (FGFR), insulin-like growth factor receptor (IGFR), insulin receptor (IR), retinoic acid receptor beta (RARβ), retinoic acid receptor gamma (RXRγ), and basic fibroblast growth factor (BFGF) in both normal and noise-damaged BP. The RT-PCR products generated were characterized by size and sequencing analysis to confirm the identities of the target molecules. The subcellular localization of the mature protein analogs for EGFR, FGFR, IGFR, RARβ, and BFGF were identified using fluorescence immunocytochemistry and confocal laser scanning microscopy. These experiments indicated that EGFR is present in the stereociliary bundles in the hair cells, IGFR is not present in the cells of the BP, BFGF localizes in the nuclei of supporting cells in the BP, but not hair cells or hyaline cells, and that RARβ localizes in the perinuclear regions of hair cells. The subcellular distributions of these proteins were consistent in both noise-damaged and control BP. FGFR, in contrast, changed its distribution in the tissue after noise damage. In normal BP, FGFR is concentrated in the stereocilia of hair cells. However, in damaged regions of noise-exposed chick cochleae, FGFR is heavily expressed in the expanded apical regions of the supporting cells. These findings suggest that BFGF and retinoic acid may potentially play a role in the mechanisms which regulate the regeneration of chicken cochlear hair cells.",
keywords = "Basic fibroblast growth factor, Chicken, Cochlea, Epidermal growth factor receptor, Fibroblast growth factor response, Hair cell, Insulin receptor, Insulin-like growth factor receptor, Regeneration, Retinoic acid, Retinoic acid receptor beta, Retinoic acid receptor gamma, Reverse transcriptase PCR",
author = "Lee, {Kenneth H.} and Cotanche, {Douglas A.}",
year = "1996",
month = "5",
doi = "10.1016/0378-5955(95)00220-0",
language = "English (US)",
volume = "94",
pages = "1--13",
journal = "Hearing Research",
issn = "0378-5955",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Potential role of bFGF and retinoic acid in the regeneration of chicken cochlear hair cells

AU - Lee, Kenneth H.

AU - Cotanche, Douglas A.

PY - 1996/5

Y1 - 1996/5

N2 - Messenger RNAs (mRNA) of several growth factor receptors and related genes were examined with reverse transcriptase polymerase chain reaction (RT-PCR) in normal and noise-damaged chicken basilar papillae (BP). Analysis of the amplification products indicated the presence of mRNAs for epidermal growth factor receptor (EGFR), fibroblast growth factor receptor (FGFR), insulin-like growth factor receptor (IGFR), insulin receptor (IR), retinoic acid receptor beta (RARβ), retinoic acid receptor gamma (RXRγ), and basic fibroblast growth factor (BFGF) in both normal and noise-damaged BP. The RT-PCR products generated were characterized by size and sequencing analysis to confirm the identities of the target molecules. The subcellular localization of the mature protein analogs for EGFR, FGFR, IGFR, RARβ, and BFGF were identified using fluorescence immunocytochemistry and confocal laser scanning microscopy. These experiments indicated that EGFR is present in the stereociliary bundles in the hair cells, IGFR is not present in the cells of the BP, BFGF localizes in the nuclei of supporting cells in the BP, but not hair cells or hyaline cells, and that RARβ localizes in the perinuclear regions of hair cells. The subcellular distributions of these proteins were consistent in both noise-damaged and control BP. FGFR, in contrast, changed its distribution in the tissue after noise damage. In normal BP, FGFR is concentrated in the stereocilia of hair cells. However, in damaged regions of noise-exposed chick cochleae, FGFR is heavily expressed in the expanded apical regions of the supporting cells. These findings suggest that BFGF and retinoic acid may potentially play a role in the mechanisms which regulate the regeneration of chicken cochlear hair cells.

AB - Messenger RNAs (mRNA) of several growth factor receptors and related genes were examined with reverse transcriptase polymerase chain reaction (RT-PCR) in normal and noise-damaged chicken basilar papillae (BP). Analysis of the amplification products indicated the presence of mRNAs for epidermal growth factor receptor (EGFR), fibroblast growth factor receptor (FGFR), insulin-like growth factor receptor (IGFR), insulin receptor (IR), retinoic acid receptor beta (RARβ), retinoic acid receptor gamma (RXRγ), and basic fibroblast growth factor (BFGF) in both normal and noise-damaged BP. The RT-PCR products generated were characterized by size and sequencing analysis to confirm the identities of the target molecules. The subcellular localization of the mature protein analogs for EGFR, FGFR, IGFR, RARβ, and BFGF were identified using fluorescence immunocytochemistry and confocal laser scanning microscopy. These experiments indicated that EGFR is present in the stereociliary bundles in the hair cells, IGFR is not present in the cells of the BP, BFGF localizes in the nuclei of supporting cells in the BP, but not hair cells or hyaline cells, and that RARβ localizes in the perinuclear regions of hair cells. The subcellular distributions of these proteins were consistent in both noise-damaged and control BP. FGFR, in contrast, changed its distribution in the tissue after noise damage. In normal BP, FGFR is concentrated in the stereocilia of hair cells. However, in damaged regions of noise-exposed chick cochleae, FGFR is heavily expressed in the expanded apical regions of the supporting cells. These findings suggest that BFGF and retinoic acid may potentially play a role in the mechanisms which regulate the regeneration of chicken cochlear hair cells.

KW - Basic fibroblast growth factor

KW - Chicken

KW - Cochlea

KW - Epidermal growth factor receptor

KW - Fibroblast growth factor response

KW - Hair cell

KW - Insulin receptor

KW - Insulin-like growth factor receptor

KW - Regeneration

KW - Retinoic acid

KW - Retinoic acid receptor beta

KW - Retinoic acid receptor gamma

KW - Reverse transcriptase PCR

UR - http://www.scopus.com/inward/record.url?scp=0030030195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030030195&partnerID=8YFLogxK

U2 - 10.1016/0378-5955(95)00220-0

DO - 10.1016/0378-5955(95)00220-0

M3 - Article

VL - 94

SP - 1

EP - 13

JO - Hearing Research

JF - Hearing Research

SN - 0378-5955

IS - 1-2

ER -