Preliminary study of the mechanism of gentamicin bound by peptidoglycan of the cell wall of Micromonospora echinospore var.

Wen ze Niu, Hui Hu, Ju Chu, You rong Li

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Abstract

Cell wall (CW) and peptidoglycan (PG) were isolated from Micromonospora echinospora. PG, the major part of cell wall, can bind about 95 % of the total GM adsorbed on the cell wall. The optimum pH appeared to be 4-9 for the binding of GM by peptidoglycan. The maximum content of bound GM was ca. 88000u/g of dry weight of peptidoglycan and no any free GM was detected in the solution. Metal ions and amine compete with GM for the binding site. Mg2+ and Na+ desorbed the binding of GM intensively at GM concentration less than 1000u/ml and more than 2000u/ml, respectively. Tris can also release GM from peptidoglycan to a certain degree.

Original languageEnglish (US)
Pages (from-to)65-69
Number of pages5
JournalChinese Journal of Antibiotics
Volume28
Issue number2
StatePublished - Jan 1 2003

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Keywords

  • Amine
  • Binding site
  • Cell wall
  • Gentamicin
  • Metal ions
  • Peptidoglycan

ASJC Scopus subject areas

  • Pharmacology

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