Abstract
We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.
Original language | English (US) |
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Pages (from-to) | 229-232 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 279 |
Issue number | 2 |
DOIs | |
State | Published - Feb 25 1991 |
Keywords
- Alternative splicing
- E2 gene
- Genomic clone
- cDNA
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology