Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex

Kim S. Lau, Jun Lee, Charles W. Fisher, Rody P. Cox, David T. Chuang

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.

Original languageEnglish (US)
Pages (from-to)229-232
Number of pages4
JournalFEBS Letters
Volume279
Issue number2
DOIs
StatePublished - Feb 25 1991

Keywords

  • Alternative splicing
  • E2 gene
  • Genomic clone
  • cDNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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