Preparation and characterization of unilamellar vesicles from cholate-phospholipid micelle treated with cholestyramine

Joseph B. Ventimiglia, Marc C. Levesque, T. Y. Chang

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Cholestyramine, a well-known bile-salt sequestrant, can be used effectively to remove cholate or deoxycholate from a solution of phosphatidylcholine-bile salt mixed micelle. Upon removal of the bile salt, unilamillar phospholipid vesicles form essentially instantaneously. Cholestyramine resin could be pelleted and removed from the vesicle solution after a low speed centrifugation. Based on phosphate analyses, the recovery of vesicles was approximately 60% of the starting material. The average diameter of these vesicles, as estimated by gel exclusion chromatography on sephacryl S-1000 beads and by trapped volume measurement using [3H]sucrose, ranged between 85 to 121 nm. Phosphatidylethanolamine, cholesterol, or n-alkane such as tetradecane can be incorporated into the vesicles without any selective loss; however, selective loss was experienced when negatively charged phospholipid species such as phosphatidylglycerol or phosphatidylserine was included in vesicle formation.

Original languageEnglish (US)
Pages (from-to)323-330
Number of pages8
JournalAnalytical biochemistry
Volume157
Issue number2
DOIs
StatePublished - Sep 1986

Keywords

  • detergent removal
  • lipid vesicles
  • model membrane

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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