Prevalence and association of the longus pilus structural gene (lngA) with colonization factor antigens, enterotoxin types, and serotypes of enterotoxigenic Escherichia coli

J. A. Giron, G. I. Viboud, V. Sperandio, O. G. Gomez-Duarte, D. R. Maneval, M. J. Albert, M. M. Levine, J. B. Kaper

Research output: Contribution to journalArticle

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Abstract

Human enterotoxigenic Escherichia coli (ETEC) produces a plasmid-encoded type IV pilus termed longus (for long pilus). Regardless of the geographic origins of ETEC strains, the longus structural gene lngA was found to have the highest level of association with ETEC producing colonization factor antigen (CFA) CFA/II, followed by ETEC producing CFA/I and CFA/IV. ETEC bearing the less prevalent CFA/III and putative colonization factors and ETEC negative for CFA and putative colonization factor also contained lngA- related sequences. lngA was found in a considerable number of ETEC serotypes and was more often associated with ETEC producing heat-stable enterotoxins than with ETEC producing both heat-labile and heat-stable enterotoxins or heat-labile enterotoxin alone. lngA was found more often in strains isolated from children with diarrhea than in strains from healthy children, suggesting on association with intestinal disease. We conclude that longus is a widely distributed antigenic determinant in ETEC that is highly associated with known plasmid-encoded virulence factors, namely, CFAs and enterotoxins. A longus-specific probe may be a helpful epidemiological tool to assist in the identification of ETEC.

Original languageEnglish (US)
Pages (from-to)4195-4198
Number of pages4
JournalInfection and Immunity
Volume63
Issue number10
StatePublished - 1995

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Enterotoxigenic Escherichia coli
Enterotoxins
Genes
Hot Temperature
Plasmids
colonization factor antigens
Serogroup
Intestinal Diseases
Virulence Factors
Epitopes
Diarrhea

ASJC Scopus subject areas

  • Immunology

Cite this

Prevalence and association of the longus pilus structural gene (lngA) with colonization factor antigens, enterotoxin types, and serotypes of enterotoxigenic Escherichia coli. / Giron, J. A.; Viboud, G. I.; Sperandio, V.; Gomez-Duarte, O. G.; Maneval, D. R.; Albert, M. J.; Levine, M. M.; Kaper, J. B.

In: Infection and Immunity, Vol. 63, No. 10, 1995, p. 4195-4198.

Research output: Contribution to journalArticle

Giron, J. A. ; Viboud, G. I. ; Sperandio, V. ; Gomez-Duarte, O. G. ; Maneval, D. R. ; Albert, M. J. ; Levine, M. M. ; Kaper, J. B. / Prevalence and association of the longus pilus structural gene (lngA) with colonization factor antigens, enterotoxin types, and serotypes of enterotoxigenic Escherichia coli. In: Infection and Immunity. 1995 ; Vol. 63, No. 10. pp. 4195-4198.
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AU - Giron, J. A.

AU - Viboud, G. I.

AU - Sperandio, V.

AU - Gomez-Duarte, O. G.

AU - Maneval, D. R.

AU - Albert, M. J.

AU - Levine, M. M.

AU - Kaper, J. B.

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