Production of endothelin-1 by rat cultured mesangial cells

Hisato Sakamoto, Sei Sasaki, Yukio Hirata, Taihei Imai, Kenji Ando, Takashi Ida, Takeshi Sakurai, Masashi Yanagisawa, Tomoh Masaki, Fumiaki Marumo

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

We investigated whether ET-1 is synthesized by and released from mesangial cells. ET-1-like immunoreactivity (LI) released into medium increased time-dependently under a serum-free condition. The amounts of ET-1-LI released into the medium was augmented in the presence of fetal calf serum. Reverse-phase HPLC profile of the conditioned media revealed a major component coeluting with standard ET-1. Northern blot analysis of poly(A)+RNA extracted from mesangial cells showed a single major band corresponding to the size of preproET-1 mRNA (2.3 kb). These findings demonstrate that ET-1 is synthesized by and released from rat mesangial cells and suggest a possibility that it acts on their own cells as an autocrine factor.

Original languageEnglish (US)
Pages (from-to)462-468
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume169
Issue number2
DOIs
StatePublished - Jun 15 1990

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Mesangial Cells
Endothelin-1
Rats
Cultured Cells
Messenger RNA
Conditioned Culture Medium
Serum
Northern Blotting
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sakamoto, H., Sasaki, S., Hirata, Y., Imai, T., Ando, K., Ida, T., ... Marumo, F. (1990). Production of endothelin-1 by rat cultured mesangial cells. Biochemical and Biophysical Research Communications, 169(2), 462-468. https://doi.org/10.1016/0006-291X(90)90354-P

Production of endothelin-1 by rat cultured mesangial cells. / Sakamoto, Hisato; Sasaki, Sei; Hirata, Yukio; Imai, Taihei; Ando, Kenji; Ida, Takashi; Sakurai, Takeshi; Yanagisawa, Masashi; Masaki, Tomoh; Marumo, Fumiaki.

In: Biochemical and Biophysical Research Communications, Vol. 169, No. 2, 15.06.1990, p. 462-468.

Research output: Contribution to journalArticle

Sakamoto, H, Sasaki, S, Hirata, Y, Imai, T, Ando, K, Ida, T, Sakurai, T, Yanagisawa, M, Masaki, T & Marumo, F 1990, 'Production of endothelin-1 by rat cultured mesangial cells', Biochemical and Biophysical Research Communications, vol. 169, no. 2, pp. 462-468. https://doi.org/10.1016/0006-291X(90)90354-P
Sakamoto, Hisato ; Sasaki, Sei ; Hirata, Yukio ; Imai, Taihei ; Ando, Kenji ; Ida, Takashi ; Sakurai, Takeshi ; Yanagisawa, Masashi ; Masaki, Tomoh ; Marumo, Fumiaki. / Production of endothelin-1 by rat cultured mesangial cells. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 169, No. 2. pp. 462-468.
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