Products of the TAL2 oncogene in leukemic T cells: bHLH phosphoproteins with DNA-binding activity

Ying Xia, Larn Yuan Hwang, Melanie H. Cobb, Richard Baer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The TAL2 gene is activated as a result of the (7;9) (q34;q32) translocation, a chromosome defect found in the malignant cells of some patients with T-cell acute lymphoblastic leukemia (T-ALL). TAL2 potentially encodes a basic helix-loop-helix motif that is highly related to those specified by TAL1 and LYL1, distinct genes that have also been implicated in T-ALL. In this report we show that leukemic cells bearing the (7;9) (q34;q32) translocation express a TAL2 gene product of 108 amino acids. In leukemic cells this product exists in both a phosphorylated (pp13(TAL2)) and an unphosphorylated (p12(TAL2)) form. Serine residue 100 is the major site of TAL2 phosphorylation in vivo, and it serves as an effective in vitro substrate for mitogen-activated protein (MAP) kinases such as ERK1. TAL2 polypeptides interact in vivo with the E2A gene products (E47 and E12) to form bHLH heterodimers that bind DNA in a sequence-specific manner. The TAL2 polypeptides do not bind DNA by themselves, however, suggesting that their functional properties may be contingent upon association with other bHLH proteins. Taken together, the properties of TAL2 evaluated here broadly resemble those described previously for TAL1, and therefore support the idea that both proteins promote T-ALL by a common mechanism.

Original languageEnglish (US)
Pages (from-to)1437-1446
Number of pages10
JournalOncogene
Volume9
Issue number5
StatePublished - May 1994

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Phosphoproteins
Oncogene Proteins
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
T-Lymphocytes
DNA
Genes
Helix-Loop-Helix Motifs
Basic Helix-Loop-Helix Transcription Factors
Peptides
Mitogen-Activated Protein Kinases
Serine
Chromosomes
Phosphorylation
Amino Acids
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Products of the TAL2 oncogene in leukemic T cells : bHLH phosphoproteins with DNA-binding activity. / Xia, Ying; Hwang, Larn Yuan; Cobb, Melanie H.; Baer, Richard.

In: Oncogene, Vol. 9, No. 5, 05.1994, p. 1437-1446.

Research output: Contribution to journalArticle

Xia, Ying ; Hwang, Larn Yuan ; Cobb, Melanie H. ; Baer, Richard. / Products of the TAL2 oncogene in leukemic T cells : bHLH phosphoproteins with DNA-binding activity. In: Oncogene. 1994 ; Vol. 9, No. 5. pp. 1437-1446.
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