Prolonged hypothermia causes primary nonfunction in preserved canine renal allografts due to humoral rejection

Barbara J. Gilligan, Heung M. Woo, Mociej Kosieradzki, Jose R. Torrealba, James H. Southard, Martin J. Mangino

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Canina kidney preservation models have historically used autotransplants to avoid the complications of rejection, although clinically all transplants are allografts. This study investigated the effects of preservation time and method on early kidney function in a canine allograft vs. autograft model. Kidneys were harvested from beagles, preserved by cold storage (CS) in UW solution for 0, 24 or 72 h, or by machine perfusion (MP) with Belzer MPS for 72 h. In some experiments 45 min of warm ischemia (WI) was performed in situ before harvest. Allograft recipients received steroid immunosuppression. Kidney function was assessed by serum creatinine and survival for 7 days. Allografts preserved for 0 and 24 h performed as well as autografts. Allografts preserved for 72 h by either CS or MP had a higher incidence of primary non function (PNF) compared with autografts, as determined by survival (50% vs. 100%, p < 0.003). Primary nonfunction kidneys had thrombotic microangiopathy, vascular and peritubular capillary binding of IgM and complement C4d, and evidence of circulating donor-specific antibodies; all consistent with humoral rejection. These responses were dependant on hypothermia time and were not attributable to ischemia, immunosuppression, preservation solution, or cellular rejection. In conclusion, prolonged hypothermia can cause PNF in allografts owing to acute humoral rejection.

Original languageEnglish (US)
Pages (from-to)1266-1273
Number of pages8
JournalAmerican Journal of Transplantation
Volume4
Issue number8
DOIs
StatePublished - Aug 2004

Fingerprint

Hypothermia
Allografts
Canidae
Autografts
Kidney
Immunosuppression
Perfusion
Thrombotic Microangiopathies
Warm Ischemia
Immunoglobulin M
Blood Vessels
Creatinine
Ischemia
Steroids
Transplants
Antibodies
Incidence
Serum

Keywords

  • Graft rejection
  • Preservation

ASJC Scopus subject areas

  • Immunology

Cite this

Prolonged hypothermia causes primary nonfunction in preserved canine renal allografts due to humoral rejection. / Gilligan, Barbara J.; Woo, Heung M.; Kosieradzki, Mociej; Torrealba, Jose R.; Southard, James H.; Mangino, Martin J.

In: American Journal of Transplantation, Vol. 4, No. 8, 08.2004, p. 1266-1273.

Research output: Contribution to journalArticle

Gilligan, Barbara J. ; Woo, Heung M. ; Kosieradzki, Mociej ; Torrealba, Jose R. ; Southard, James H. ; Mangino, Martin J. / Prolonged hypothermia causes primary nonfunction in preserved canine renal allografts due to humoral rejection. In: American Journal of Transplantation. 2004 ; Vol. 4, No. 8. pp. 1266-1273.
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