Prospective application of activity-based proteomic profiling in vision research-potential unique insights into ocular protease biology and pathology

Hui Peng, John D Hulleman

Research output: Contribution to journalReview article

Abstract

Activity-based proteomic profiling (ABPP) is a powerful tool to specifically target and measure the activity of a family of enzymes with the same function and reactivity, which provides a significant advantage over conventional proteomic strategies that simply provide abundance information. A number of inherited and age-related eye diseases are caused by polymorphisms/mutations or abnormal expression of proteases including serine proteases, cysteine proteases, and matrix metalloproteinases, amongst others. However, neither conventional genomic, transcriptomic, nor traditional proteomic profiling directly interrogate protease activities. Thus, leveraging ABPP to probe the activity of these enzyme classes as they relate to normal function and pathophysiology of the eye represents a unique potential opportunity for disease interrogation and possibly intervention.

Original languageEnglish (US)
Article number3855
JournalInternational journal of molecular sciences
Volume20
Issue number16
DOIs
StatePublished - Aug 2 2019

Fingerprint

protease
pathology
Pathology
biology
Proteomics
Peptide Hydrolases
Research
Enzymes
enzymes
eye diseases
Cysteine Proteases
Eye Diseases
Serine Proteases
Polymorphism
Matrix Metalloproteinases
interrogation
cysteine
polymorphism
mutations
reactivity

Keywords

  • Age-related macular degeneration
  • Chemical biology
  • Enzyme activity
  • Eye disease
  • Proteases
  • Retinal degeneration

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

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abstract = "Activity-based proteomic profiling (ABPP) is a powerful tool to specifically target and measure the activity of a family of enzymes with the same function and reactivity, which provides a significant advantage over conventional proteomic strategies that simply provide abundance information. A number of inherited and age-related eye diseases are caused by polymorphisms/mutations or abnormal expression of proteases including serine proteases, cysteine proteases, and matrix metalloproteinases, amongst others. However, neither conventional genomic, transcriptomic, nor traditional proteomic profiling directly interrogate protease activities. Thus, leveraging ABPP to probe the activity of these enzyme classes as they relate to normal function and pathophysiology of the eye represents a unique potential opportunity for disease interrogation and possibly intervention.",
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