Protection from T helper cell-mediated graft-versus-host disease by the presence of an MHC class I alloantigen is associated with perturbation of MHC class II-restricted responses by class I-derived peptides

Ramona R. Leibnitz, Peter E. Lipsky, Dwain L Thiele

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Lethal graft-vs-host disease (GVHD) develops after transfer of CTL- depleted spleen and bone marrow cells from C57BL/6 (B6) mice to irradiated MHC class II disparate (B6xbm12)F1 recipients. Onset of lethal GVHD is significantly delayed in (bm1xbm12)F1 recipients of the same donor inoculum despite the additional MHC class I disparity (H-2K(bm1)). To investigate the basis of this protective effect, hybridomas were generated from T cells activated in vivo during GVHD in both strain combinations. T cells from B6→(B6xbm12)F1 mice generated a significantly higher frequency of B6.C-H- 2(bm12) (bm12)-specific T hybridomas (110/178, 62%) than T cells from B6→(bm1xbm12)F1 mice (102/218, 47%). Some bm12-specific T hybridomas exhibited lesser responses to (bm1xbm12)F1 than to (B6xbm12)F1 APC. Moreover, bm1 peptides spanning a 14-amino acid region that includes the three amino acids that differ from H-2K(b) were found to inhibit responses of some bm12-specific T hybridomas and to decrease significantly responses of splenic B6 CD4+ T cells to bm12 APC. Notably, the frequency of bm12-reactive hybridomas susceptible to inhibition by bm1 peptides generated from B6→(B6xbm12)F1 mice was significantly greater than that generated from B6→(bm1xbm12)F1 mice. Additional analysis using L cell transfectants indicated that hybridomas responding to the bm12 mutation at position 70 alone were rarely inhibited by bm1 peptides. The data indicate that expression of bm1-derived peptides can influence the frequency and specificity of alloreactive CD4+ T cells stimulated in vivo and thus may alter the course of GVHD.

Original languageEnglish (US)
Pages (from-to)1784-1795
Number of pages12
JournalJournal of Immunology
Issue number4
StatePublished - Jan 1 1995


ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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