Protein structure prediction for the male-specific region of the human Y chromosome

Krzysztof Ginalski, Leszek Rychlewski, David Baker, Nick V. Grishin

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

The complete sequence of the male-specific region of the human Y chromosome (MSY) has been determined recently; however, detailed characterization for many of its encoded proteins still remains to be done. We applied state-of-the-art protein structure prediction methods to all 27 distinct MSY-encoded proteins to provide better understanding of their biological functions and their mechanisms of action at the molecular level. The results of such large-scale structure-functional annotation provide a comprehensive view of the MSY proteome, shedding light on MSY-related processes. We found that, in total, at least 60 domains are encoded by 27 distinct MSY genes, of which 42 (70%) were reliably mapped to currently known structures. The most challenging predictions include the unexpected but confident 3D structure assignments for three domains identified here encoded by the USP9Y, UTY, and BPY2 genes. The domains with unknown 3D structures that are not predictable with currently available theoretical methods are established as primary targets for crystallographic or NMR studies. The data presented here set up the basis for additional scientific discoveries in human biology of the Y chromosome, which plays a fundamental role in sex determination.

Original languageEnglish (US)
Pages (from-to)2305-2310
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number8
DOIs
StatePublished - Feb 22 2004

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Protein structure prediction for the male-specific region of the human Y chromosome'. Together they form a unique fingerprint.

  • Cite this