Proteins that control the geometry of microtubules at the ends of cilia

Panagiota Louka, Krishna Kumar Vasudevan, Mayukh Guha, Ewa Joachimiak, Dorota Wloga, Raphaël F.X. Tomasi, Charles N. Baroud, Pascale Dupuis‑Williams, Domenico F. Galati, Chad G. Pearson, Luke M Rice, James J. Moresco, John R. Yates, Yu Yang Jiang, Karl Lechtreck, William Dentler, Jacek Gaertig

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6 Scopus citations

Abstract

Cilia, essential motile and sensory organelles, have several compartments: the basal body, transition zone, and the middle and distal axoneme segments. The distal segment accommodates key functions, including cilium assembly and sensory activities. While the middle segment contains doublet microtubules (incomplete B-tubules fused to complete A-tubules), the distal segment contains only A-tubule extensions, and its existence requires coordination of microtubule length at the nanometer scale. We show that three conserved proteins, two of which are mutated in the ciliopathy Joubert syndrome, determine the geometry of the distal segment, by controlling the positions of specific microtubule ends. FAP256/CEP104 promotes A-tubule elongation. CHE-12/Crescerin and ARMC9 act as positive and negative regulators of B-tubule length, respectively. We show that defects in the distal segment dimensions are associated with motile and sensory deficiencies of cilia. Our observations suggest that abnormalities in distal segment organization cause a subset of Joubert syndrome cases.

Original languageEnglish (US)
Pages (from-to)4298-4313
Number of pages16
JournalJournal of Cell Biology
Volume217
Issue number12
DOIs
StatePublished - Dec 1 2018

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ASJC Scopus subject areas

  • Cell Biology

Cite this

Louka, P., Vasudevan, K. K., Guha, M., Joachimiak, E., Wloga, D., Tomasi, R. F. X., Baroud, C. N., Dupuis‑Williams, P., Galati, D. F., Pearson, C. G., Rice, L. M., Moresco, J. J., Yates, J. R., Jiang, Y. Y., Lechtreck, K., Dentler, W., & Gaertig, J. (2018). Proteins that control the geometry of microtubules at the ends of cilia. Journal of Cell Biology, 217(12), 4298-4313. https://doi.org/10.1083/jcb.201804141