Proteomic analysis of sera from hepatocellular carcinoma patients after radiofrequency ablation treatment

Takayuki Kawakami; Yujin Hoshida; Fumihiko Kanai; Yasuo Tanaka; Keisuke Tateishi; Tsuneo Ikenoue; Shuntaro Obi; Shinpei Sato; Takuma Teratani; Shuichiro Shiina; Takao Kawabe; Takamasa Suzuki; Naoya Hatano; Hisaaki Taniguchi; Masao Omata

doi:10.1002/pmic.200401287

Proteomic analysis of sera from hepatocellular carcinoma patients after radiofrequency ablation treatment

Takayuki Kawakami, Yujin Hoshida, Fumihiko Kanai, Yasuo Tanaka, Keisuke Tateishi, Tsuneo Ikenoue, Shuntaro Obi, Shinpei Sato, Takuma Teratani, Shuichiro Shiina, Takao Kawabe, Takamasa Suzuki, Naoya Hatano, Hisaaki Taniguchi, Masao Omata

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

Comparative proteomic analysis was used to search for characteristic alterations in the sera of hepatocellular carcinoma (HCC) patients who had undergone curative radiofrequency ablation treatment. Serum samples collected from eight patients before and after treatment were subjected to 2-DE. Eighty-eight protein spots differentially expressed with the treatment were selected by clustering analysis, and the proteins were identified by MS based on MALDI-TOF/TOF analysis and public database searches. The statistical analysis suggested that four proteins decreased after treatment (pro-apolipoprotein, α2-HS glycoprotein, apolipoprotein A-IV precursor, and PRO1708/PRO2044, which is the carboxy terminal fragment of albumin) and that seven proteins were increased after treatment, including leucine-rich α2-glycoprotein and α1-antitrypsin. These data facilitate the identification of differentially expressed proteins that are involved in HCC carcinogenesis and provide candidate biomarkers for the development of diagnostic and therapeutic tools.

Original language	English (US)
Pages (from-to)	4287-4295
Number of pages	9
Journal	Proteomics
Volume	5
Issue number	16
DOIs	https://doi.org/10.1002/pmic.200401287
State	Published - Nov 2005
Externally published	Yes

Keywords

2-DE
Hepatocellular carcinoma
Serum proteome

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1002/pmic.200401287

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title = "Proteomic analysis of sera from hepatocellular carcinoma patients after radiofrequency ablation treatment",

abstract = "Comparative proteomic analysis was used to search for characteristic alterations in the sera of hepatocellular carcinoma (HCC) patients who had undergone curative radiofrequency ablation treatment. Serum samples collected from eight patients before and after treatment were subjected to 2-DE. Eighty-eight protein spots differentially expressed with the treatment were selected by clustering analysis, and the proteins were identified by MS based on MALDI-TOF/TOF analysis and public database searches. The statistical analysis suggested that four proteins decreased after treatment (pro-apolipoprotein, α2-HS glycoprotein, apolipoprotein A-IV precursor, and PRO1708/PRO2044, which is the carboxy terminal fragment of albumin) and that seven proteins were increased after treatment, including leucine-rich α2-glycoprotein and α1-antitrypsin. These data facilitate the identification of differentially expressed proteins that are involved in HCC carcinogenesis and provide candidate biomarkers for the development of diagnostic and therapeutic tools.",

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author = "Takayuki Kawakami and Yujin Hoshida and Fumihiko Kanai and Yasuo Tanaka and Keisuke Tateishi and Tsuneo Ikenoue and Shuntaro Obi and Shinpei Sato and Takuma Teratani and Shuichiro Shiina and Takao Kawabe and Takamasa Suzuki and Naoya Hatano and Hisaaki Taniguchi and Masao Omata",

year = "2005",

month = nov,

doi = "10.1002/pmic.200401287",

language = "English (US)",

volume = "5",

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journal = "Proteomics",

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T1 - Proteomic analysis of sera from hepatocellular carcinoma patients after radiofrequency ablation treatment

AU - Kawakami, Takayuki

AU - Hoshida, Yujin

AU - Kanai, Fumihiko

AU - Tanaka, Yasuo

AU - Tateishi, Keisuke

AU - Ikenoue, Tsuneo

AU - Obi, Shuntaro

AU - Sato, Shinpei

AU - Teratani, Takuma

AU - Shiina, Shuichiro

AU - Kawabe, Takao

AU - Suzuki, Takamasa

AU - Hatano, Naoya

AU - Taniguchi, Hisaaki

AU - Omata, Masao

PY - 2005/11

Y1 - 2005/11

N2 - Comparative proteomic analysis was used to search for characteristic alterations in the sera of hepatocellular carcinoma (HCC) patients who had undergone curative radiofrequency ablation treatment. Serum samples collected from eight patients before and after treatment were subjected to 2-DE. Eighty-eight protein spots differentially expressed with the treatment were selected by clustering analysis, and the proteins were identified by MS based on MALDI-TOF/TOF analysis and public database searches. The statistical analysis suggested that four proteins decreased after treatment (pro-apolipoprotein, α2-HS glycoprotein, apolipoprotein A-IV precursor, and PRO1708/PRO2044, which is the carboxy terminal fragment of albumin) and that seven proteins were increased after treatment, including leucine-rich α2-glycoprotein and α1-antitrypsin. These data facilitate the identification of differentially expressed proteins that are involved in HCC carcinogenesis and provide candidate biomarkers for the development of diagnostic and therapeutic tools.

AB - Comparative proteomic analysis was used to search for characteristic alterations in the sera of hepatocellular carcinoma (HCC) patients who had undergone curative radiofrequency ablation treatment. Serum samples collected from eight patients before and after treatment were subjected to 2-DE. Eighty-eight protein spots differentially expressed with the treatment were selected by clustering analysis, and the proteins were identified by MS based on MALDI-TOF/TOF analysis and public database searches. The statistical analysis suggested that four proteins decreased after treatment (pro-apolipoprotein, α2-HS glycoprotein, apolipoprotein A-IV precursor, and PRO1708/PRO2044, which is the carboxy terminal fragment of albumin) and that seven proteins were increased after treatment, including leucine-rich α2-glycoprotein and α1-antitrypsin. These data facilitate the identification of differentially expressed proteins that are involved in HCC carcinogenesis and provide candidate biomarkers for the development of diagnostic and therapeutic tools.

KW - 2-DE

KW - Hepatocellular carcinoma

KW - Serum proteome

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ER -

Proteomic analysis of sera from hepatocellular carcinoma patients after radiofrequency ablation treatment

Abstract

Keywords

ASJC Scopus subject areas

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