Psoriatic epidermal cells demonstrate increased numbers and function of non-Langerhans antigen-presenting cells

Ole Baadsgaard, Aditya K. Gupta, R. Stanley Taylor, Charles N. Ellis, John J. Voorhees, Kevin D. Cooper

Research output: Contribution to journalArticle

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Abstract

The recent findings that the immunosuppressant cyclosporine A (CsA) improves psoriasis raises the possibility that cellular immune processes play a major role in the pathogenesis of psoriasis. We therefore investigated the phenotype and function of cells within psoriatic epidermis that can play a role in cellular immunologic reactivity. Double fluorescence microscopic studies with monoclonal antibodies of epidermal cells in suspension (EC) and of histologic sections demonstrated that involved psoriatic skin contained a significantly increased number of non-Langerhans cell T6-DR+ EC (4.9 + 2.1%) relative to uninvolved (0.3 ±0.1%), p < 0.01. This non-Langerhans cell population was comprised of DR+ monocytes, DR+ activated T lymphocytes, a few DR+RFD1+ antigen-presenting cells (APC), and DR+ ke ratinocytes. Langerhans cell (LC) levels in EC suspension were not different between involved and uninvolved psoriatic epidermis. Functional studies demonstrated that involved psoriatic epidermal cells had an increased capacity to induce T-cell activation and proliferation relative to uninvolved EC (p< 0.04). This increased APC activity was due to the non-LC T6-DR+HLe1+ APC population and not to DR+ keratinocytes. These results demonstrate that involved psoriatic epidermal cells contain both an increased number and function of antigen-presenting cells. The pathogenetic mechanisms in psoriasis may be related to ongoing cellular immune responses in the skin, and the effect of CsA may be mediated through a suppressive effect on the enhanced antigen-presenting cell activity.

Original languageEnglish (US)
Pages (from-to)190-195
Number of pages6
JournalJournal of Investigative Dermatology
Volume92
Issue number2
StatePublished - Feb 1989

Fingerprint

Antigen-Presenting Cells
T-cells
Psoriasis
Cyclosporine
Suspensions
Skin
Epidermis
Immunosuppressive Agents
T-Lymphocytes
Langerhans Cells
Fluorescence
Chemical activation
Monoclonal Antibodies
Cells
Keratinocytes
Cellular Immunity
Population
Monocytes
Cell Proliferation
Phenotype

ASJC Scopus subject areas

  • Dermatology

Cite this

Baadsgaard, O., Gupta, A. K., Stanley Taylor, R., Ellis, C. N., Voorhees, J. J., & Cooper, K. D. (1989). Psoriatic epidermal cells demonstrate increased numbers and function of non-Langerhans antigen-presenting cells. Journal of Investigative Dermatology, 92(2), 190-195.

Psoriatic epidermal cells demonstrate increased numbers and function of non-Langerhans antigen-presenting cells. / Baadsgaard, Ole; Gupta, Aditya K.; Stanley Taylor, R.; Ellis, Charles N.; Voorhees, John J.; Cooper, Kevin D.

In: Journal of Investigative Dermatology, Vol. 92, No. 2, 02.1989, p. 190-195.

Research output: Contribution to journalArticle

Baadsgaard, O, Gupta, AK, Stanley Taylor, R, Ellis, CN, Voorhees, JJ & Cooper, KD 1989, 'Psoriatic epidermal cells demonstrate increased numbers and function of non-Langerhans antigen-presenting cells', Journal of Investigative Dermatology, vol. 92, no. 2, pp. 190-195.
Baadsgaard O, Gupta AK, Stanley Taylor R, Ellis CN, Voorhees JJ, Cooper KD. Psoriatic epidermal cells demonstrate increased numbers and function of non-Langerhans antigen-presenting cells. Journal of Investigative Dermatology. 1989 Feb;92(2):190-195.
Baadsgaard, Ole ; Gupta, Aditya K. ; Stanley Taylor, R. ; Ellis, Charles N. ; Voorhees, John J. ; Cooper, Kevin D. / Psoriatic epidermal cells demonstrate increased numbers and function of non-Langerhans antigen-presenting cells. In: Journal of Investigative Dermatology. 1989 ; Vol. 92, No. 2. pp. 190-195.
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