QseC mediates Salmonella enterica serovar typhimurium virulence in vitro and in vivo

Cristiano G. Moreira, David Weinshenker, Vanessa Sperandio

Research output: Contribution to journalArticle

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Abstract

The autoinducer-3 (AI-3)/epinephrine (Epi)/norepinephrine (NE) interkingdom signaling system mediates chemical communication between bacteria and their mammalian hosts. The three signals are sensed by the QseC histidine kinase (HK) sensor. Salmonella enterica serovar Typhimurium is a pathogen that uses HKs to sense its environment and regulate virulence. Salmonella serovar Typhimurium invades epithelial cells and survives within macrophages. Invasion of epithelial cells is mediated by the type III secretion system (T3SS) encoded in Salmonella pathogenicity island 1 (SPI-1), while macrophage survival and systemic disease are mediated by the T3SS encoded in SPI-2. Here we show that QseC plays an important role in Salmonella serovar Typhimurium pathogenicity. A qseC mutant was impaired in flagellar motility, in invasion of epithelial cells, and in survival within macrophages and was attenuated for systemic infection in 129x1/SvJ mice. QseC acts globally, regulating expression of genes within SPI-1 and SPI-2 in vitro and in vivo (during infection of mice). Additionally, dopamine β-hydroxylase knockout (Dbh-/-) mice that do not produce Epi or NE showed different susceptibility to Salmonella serovar Typhimurium infection than wild-type mice. These data suggest that the AI-3/Epi/NE signaling system is a key factor during Salmonella serovar Typhimurium pathogenesis in vitro and in vivo. Elucidation of the role of this interkingdom signaling system in Salmonella serovar Typhimurium should contribute to a better understanding of the complex interplay between the pathogen and the host during infection.

Original languageEnglish (US)
Pages (from-to)914-926
Number of pages13
JournalInfection and Immunity
Volume78
Issue number3
DOIs
StatePublished - Mar 2010

Fingerprint

Salmonella enterica
Virulence
Salmonella typhimurium
Epinephrine
Genomic Islands
Norepinephrine
Epithelial Cells
Macrophages
Infection
Salmonella
Mixed Function Oxygenases
Knockout Mice
In Vitro Techniques
Serogroup
Dopamine
Cell Survival
Bacteria
Gene Expression

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

QseC mediates Salmonella enterica serovar typhimurium virulence in vitro and in vivo. / Moreira, Cristiano G.; Weinshenker, David; Sperandio, Vanessa.

In: Infection and Immunity, Vol. 78, No. 3, 03.2010, p. 914-926.

Research output: Contribution to journalArticle

Moreira, Cristiano G. ; Weinshenker, David ; Sperandio, Vanessa. / QseC mediates Salmonella enterica serovar typhimurium virulence in vitro and in vivo. In: Infection and Immunity. 2010 ; Vol. 78, No. 3. pp. 914-926.
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