Quantitation of β-lapachone and 3-hydroxy-β-lapachone in human plasma samples by reversed-phase high-performance liquid chromatography

V. L. Glen, P. R. Hutson, N. J. Kehrli, D. A. Boothman, G. Wilding

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

β-Lapachone is an o-naphthoquinone found to have in vitro cytotoxicity in cancer cells, type I human immunodeficiency virus, and fungi. Analytical methods for evaluating β-lapachone in biological fluids using high-performance liquid chromatography (HPLC) have not been published. The reversed-phase HPLC method described in this report utilizes liquid extraction of a 0.5-ml plasma sample with average recoveries of 67 ± 10.8% and 70 ± 10.3% for β-lapachone and 3-hydroxy-β-lapachone, respectively. Sensitivity of the assay using ultraviolet (UV) detection at 256 nm is 15 ng ml-1 from a 100 μl injection. Plasma standards for β-lapaehone and 3-hydroxy-β-lapachone are linear with no significant difference in slope between the compounds. The retention times of 2.7 min for 3-hydroxy-β-lapachone and 5.9 min for β-lapachone result in a clean separation permitting use of the same assay procedure without modification for both compounds. This assay offers the advantage that either β-lapachone or 3-hydroxy-β-lapachone can serve as the internal standard, depending on which compound is being analyzed.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume692
Issue number1
DOIs
StatePublished - Apr 25 1997

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Plasma (human)
High performance liquid chromatography
Assays
Plasmas
Cytotoxicity
Fungi
Viruses
Cells
Recovery
Fluids
Liquids

Keywords

  • β-Lapachone
  • 3-Hydroxy-β-lapachone

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Quantitation of β-lapachone and 3-hydroxy-β-lapachone in human plasma samples by reversed-phase high-performance liquid chromatography. / Glen, V. L.; Hutson, P. R.; Kehrli, N. J.; Boothman, D. A.; Wilding, G.

In: Journal of Chromatography B: Biomedical Applications, Vol. 692, No. 1, 25.04.1997, p. 181-186.

Research output: Contribution to journalArticle

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